Effects of Extracellular adenosine-5'-triphosphate on Cellular damage, H2O2 Content,and Activities of H2O2 Detoxifying Enzymes under Lead Stress

doi:10.7525/j.issn.1673-5102.2020.01.013

Abstract

Abstract: The extracellular adenosine-5'-triphosphate is an important signaling molecule for plant cells. By using the tobacco(Nicotiana tabacum L.cv.Bright Yellow-2) cell suspension cultures, we studied the effects of extracellular adenosine-5'-triphosphate on the cellular damage, H₂O content, and the activities of H₂O detoxifying enzymes underlead stress. With the increase of concentrations of Pb(NO₃)₂(from 30-400 μmol·L^-1), the levels of the extracellular adenosine-5'-triphosphate were decreased, whilethe levels of the intracellular adenosine-5'-triphosphate and cellular damage were increased. The content of H₂O₂ and the activity of the CAT(catalaseenzyme) were increased and reached their maximum at 200 μmol·L^-1Pb(NO₃)₂, while the activityof the POD(peroxide enzyme) was decreased, with the increase of concentrations of Pb(NO₃)₂. Compared with the cells under lead(200 μmol·L^-1) stress, the lead-stressed cells treated with exogenous adenosine-5'-triphosphate(30 μmol·L^-1) had lower levels of cellular damage, H₂O₂ content, andthe activity of CAT, but had higher level of the activity of POD. Therefore, the cellular damage, H₂O content, and the activities of H₂O detoxifying enzymesunderlead stress could beregulated by extracellular adenosine-5'-triphosphate.

Key words: cellular damage, extracellular adenosine-5'-triphosphate, lead stress, H₂O₂, H₂O detoxifying enzymes

CLC Number:

Q945.79

CAO Jia-Xin, DA Meng-Ting, PANG Hai-Long, JIA Ling-Yun, FENG Han-Qing. Effects of Extracellular adenosine-5'-triphosphate on Cellular damage, H₂O₂ Content,and Activities of H₂O₂ Detoxifying Enzymes under Lead Stress[J]. Bulletin of Botanical Research, 2020, 40(1): 85-92.

References

[1] Nemati K,Bakar N K A,Abas M R,et al.Speciation of heavy metals by modified BCR sequential extraction procedure in different depths of sediments from Sungai Buloh,Selangor,Malaysia[J].Journal of Hazardous Materials,2011,192(1):402-410.
[2] Nicholls A M,Mal T K.Effects of lead and copper exposure on growth of an invasive weed,Lythrum salicaria L.(Purple loosestrife)[J].Ohio Journal Science,2003,103(5):129-133.
[3] Fahr M,Laplaze L,Bendaou N,et al.Effect of lead on root growth[J].Frontiers in Plant Science,2013,4:175.
[4] Pourrut B,Shahid M,Dumat C,et al.Lead uptake,toxicity,and detoxification in plants[M].//Whitacre D M.Reviews of environmental contamination and toxicology volume 213.New York:Springer,2011:113-136.
[5] Gill M.Heavy metal stress in plants:a review[J].International Journal of Advanced Research,2014,2(6):1043-1055.
[6] Wierzbicka M.The effect of lead on the cell cycle in the root meristem of Allium cepa L.[J].Protoplasma,1999,207(3-4):186-194.
[7] 张娜,杨双,童非,等.铅污染对不同生境芦苇体内抗氧化酶系统的影响[J].江苏农业学报,2018,34(2):333-339.Zhang N,Yang S,Tong F,et al.The effects of Pb pollution on the antioxidant enzyme system of Phragmites australis(common reed) in different habits[J].Jiangsu Journal of Agricultural Sciences,2018,34(2):333-339.
[8] Khakh B S,Burnstock G.The double life of ATP[J].Scientific American,2009,301(6):84-92.
[9] Burnstock G,Kennedy C.Is there a basis for distinguishing two types of P₂-purinoceptor?[J].General Pharmacology:The Vascular System,1985,16(5):433-440.
[10] Kim S Y,Sivaguru M,Stacey G.Extracellular ATP in plants.Visualization,Localization,and analysis of physiological significance in growth and signaling[J].Plant Physiology,2006,142(3):984-992.
[11] Thomas C,Rajagopal A,Windsor B,et al.A role for ectophosphatase in xenobiotic resistance[J].The Plant Cell,2000,12(4):519-533.
[12] Tanaka K,Gilroy S,Jones A M,et al.Extracellular ATP signaling in plants[J].Trends in Cell Biology,2010,20(10):601-608.
[13] Dichmann S,Idzko M,Zimpfer U,et al.Adenosine triphosphate-induced oxygenradical production and CD11b up-regulation:Ca⁺⁺ mobilization and actin reorganization in human eosinophils[J].Blood,2000,95(3):973-978.
[14] Demidchik V,Shang Z L,Shin R,et al.Plant extracellular ATP signalling by plasma membrane NADPH oxidase and Ca²⁺ channels[J].The Plant Journal,2009,58(6):903-913.
[15] Song C J,Steinebrunner I,Wang X Z,et al.Extracellular ATP induces the accumulation of superoxide via NADPH oxidases in Arabidopsis[J].Plant Physiology,2006,140(4):1222-1232.
[16] Choi J,Tanaka K,Cao Y R,et al.Identification of a plant receptor for extracellular ATP[J].Science,2014,343(6168):290-294.
[17] 白晶月,冯汉青,戎福虎,等.细胞外ATP调节NaCl胁迫诱导的烟草悬浮细胞死亡和呼吸抑制[J].四川大学学报:自然科学版,2017,54(1):191-196.Bai J Y,Feng H Q,Rong F H,et al.The studies of cell death and respiration inhibition of tobacco suspension cells under NaCl stress mediated by extracellular ATP[J].Journal of Sichuan University:Natural Science Edition,2017,54(1):191-196.
[18] Murashige T,Skoog F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Physiologia Plantarum,1962,15(3):473-497.
[19] Yang S W,Kim S K,Kim W T.Perturbation of NgTRF3 expression induces apoptosis-like cell death in tobacco BY-2 cells and implicates NgTRF1 in the control of telomere length and stability[J].The Plant Cell,2004,16(12):3370-3385.
[20] Jones K H,Senft J A.An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide[J].Journal of Histochemistry & Cytochemistry,1985,33(1):77-79.
[21] Mcelroy W D,Strehler B L.Factors influencing the response of the bioluminescent reaction to adenosine triphosphate[J].Archives of Biochemistry,1949,22(3):420-433.
[22] Dat J,Vandenabeele S,Vranová E,et al.Dual action of the active oxygen species during plant stress responses[J].Cellular and Molecular Life Sciences CMLS,2000,57(5):779-795.
[23] 耶兴元,刘建军,刘磊.H₂O₂诱导低温胁迫下草莓苗抗冷性研究[J].北方园艺,2011,(17):47-49.Ye X Y,Liu J J,Liu L.H₂O₂ induced chilling resistance of strawberry seedlings under low temperature stress[J].Northern Horticulture,2011,(17):47-49.
[24] Orozco-Cárdenas M L,Narváez-Vásquez J,Ryan C A.Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding,systemin,and methyl jasmonate[J].The Plant Cell,2001,13(1):179-191.
[25] Blokhina G,Virolainen E,Fagerstedt K V.Antioxidants,oxidative damage and oxygen deprivation stress:a review[J].Annals of Botany,2003,91(2):179-194.
[26] Mckersie B D,Leshem Y Y.Stress and stress coping in cultivated plants[M].Dordrecht:Springer,1994.
[27] 黄金秋,胡明华,廖鹏飞,等.粳稻95122A和95122B悬浮系建立及抗氧化性分析[J].基因组学与应用生物学,2015,34(12):2757-2762.Huang J Q,Hu M H,Liao P F,et al.Establishment and antioxidation ability of suspension cell lines of Japonica rice 95122A and 95122B[J].Genomics and Applied Biology,2015,34(12):2757-2762.
[28] Comba M E,Benavides M P,Tomaro M L.Effect of salt stress on antioxidant defence system in soybean root nodules[J].Australian Journal of Plant Physiology,1998,25(6):665-671.
[29] Foyer C H,Descourvièresp,Kunert K J.Protection against oxygen radicals:an important defence mechanism studied in transgenic plants[J].Plant,Cell &Environment,1994,17(5):507-523.
[30] Lösch R.Plant mitochondrial respiration under the influence of heavy metals[M].//Prasadm N V.Heavy metal stress in plants:from biomolecules to ecosystems.Berlin,Heidelberg:Springer,2004:182-200.
[31] Chivasa S,Ndimba B K,Simon W J,et al.Extracellular ATP functions as an endogenous external metabolite regulating plant cell viability[J].The Plant Cell,2005,17(11):3019-3034.

Effects of Extracellular adenosine-5'-triphosphate on Cellular damage, H₂O₂ Content,and Activities of H₂O₂ Detoxifying Enzymes under Lead Stress

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	LIU Jian-Xin, OU Xiao-Bin, WANG Jin-Cheng. Effects of Exogenous Hydrogen Peroxide on Growth and Resistance Physiology of Naked Oat Seedlings under Saline-alkali Mixed Stress [J]. Bulletin of Botanical Research, 2019, 39(2): 181-191.
[2]	LIU Jian-Xin, OU Xiao-Bin, LIU Xiu-Li, WANG Jin-Cheng. Principal Component and Subordinate Function of the Alleviating Effects of Hydrogen Peroxide(H₂O₂) on Low-temperature Stress in Naked Oat(Avena nuda) Seedlings [J]. Bulletin of Botanical Research, 2018, 38(5): 748-756.
[3]	LIU Jian-Xin, LIU Xiu-Li, WANG Jin-Cheng, JIA Hai-Yan. Effect of Ca²⁺ on Seed Germination and Seedling Formation of Oat Induced by H₂O₂ under Saline-Alkali Complex Stress [J]. Bulletin of Botanical Research, 2017, 37(6): 855-860.
[4]	TIAN Wu-Ying, JIAO Qing-Song, SHI Dai-Long, SUN Kun, JIA Ling-Yun, FENG Han-Qing. Extracellular ATP Exert Systemic Effects on Photosystem Ⅱ Photochemistry of Kidney Bean(Phaseolus vulgaris) Leaf through a H₂O₂ Signalling Pathway [J]. Bulletin of Botanical Research, 2016, 36(3): 368-373.
[5]	FENG Han-Qing；BAI Jing-Yue；GUAN Dong-Dong；JIA Ling-Yun；SUN Kun. Extracellular H₂O₂ and NADPH Oxidase are Involved in the Copper-Induced Cell Death [J]. Bulletin of Botanical Research, 2015, 35(5): 710-715.
[6]	QIU Zong-Bo;SUN Li;LI Jin-Ting;WEI Gai-Gai;YUE Ming. Protecting Effect of Exogenous Hydrogen Peroxide on Wheat Seedlings Damage by Water Stress [J]. Bulletin of Botanical Research, 2010, 30(3): 294-298.
[7]	GAO Hong-Ming;WU Xiao-Xia;ZHANG Biao;ZHANG Yi;XU Ping;SUN Guo-Rong. Changes of defensive enzymes and active oxygen of Puccinellia tenuiflora* seedlings under Na₂CO₃ stress [J]. Bulletin of Botanical Research, 2006, 26(3): 308-312.