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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (4): 635-640.doi: 10.7525/j.issn.1673-5102.2017.04.021

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Optimization of Ultrasonic Extraction of Polysaccharides from Tara Seed and Its Antioxidant Activity

ZHAO Han-Mei, YUAN De-Cheng, YANG Feng-Jian   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2017-02-05 Online:2017-07-15 Published:2017-07-22
  • Supported by:
    Forestry Public Welfare Industry Research Special(201404616)

Abstract: For the optimum ultrasonic extraction of Tara polysaccharide,a multiple quadratic regression was stablished using a 4-factor, 3-level Box-Behnken statistical design, and the response surface with the extraction rate of polysaccharides as the response were drawn. The antioxidant activities of Tara polysaccharide invitrowere tested. Theoptimumextractionconditions of Tara polysaccharide were:ultrasonic treatment at 56℃ for 23 min with ultrasonic power 363 W, and material to liquid ratio of 1:17 g·mL-1. The maximum extraction rate of Tara crude polysaccharides was 25.56%, less than the theoretical deduced value of 25.71%.The purified Tara polysaccharide extraction rate was 17.82%.Using VC as a positive control, the antioxidant activity of different purity Tara polysaccharide was measured, and it was found that its purity was high, and the ABTS radical scavenging ability was stronger.

Key words: Tara polysaccharide, extraction, response surface analysis methodology (RSM), antioxidant activity

CLC Number: