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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (5): 789-796.doi: 10.7525/j.issn.1673-5102.2017.05.020

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Ultra Turrax-Microwave Assisted Extraction of Oil from Pinus koraiensis Sieb Seed and its Quality Evaluation

YANG Ming-Fei1, SU Wen2, KOU Ping3, WANG Xi-Qing3, GUO Na3, ZANG Yu-Ping3, FU Yu-Jie3   

  1. 1. Research and Development Department of Kitanihon Pharmaceutical CO., No. 55, Kamiichi, Nakaniikawa District, Toyama, Japan 930-0314;
    2. Management Center of Public Medical and Health Institutions in Doumen District, Zhuhai City, Guangdong Province, Zhuhai 519125;
    3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2017-03-07 Online:2017-09-15 Published:2017-09-15
  • Supported by:
    13th Five-Year The National Key Research Projects(2016YFD0600805)

Abstract: In this work, ultra turrax-microwave assisted extraction process was conducted to extract oil from Pinus koraiensis using ethanol as the extraction solvent. The effects of homogenization speed, homogenization time, liquid-solid ratio, microwave temperature, microwave power, and microwave time on the oil yield were investigated. The optimal conditions for the oil extraction from P.koraiensis seed were obtained as follows:homogeneous speed 12 000 r·min-1, homogeneous time 120 s, the ratio of liquid to material 20 mL·g-1, microwave temperature 60℃, microwave power 700 W and microwave time 50 min. Under the above conditions, the optimum oil yield was 60.3%. The fatty acid composition of P.koraiensis seed oil was analyzed by GC-MS, and the main components were identified as linoleic acid, stearic acid and palmitic acid. The contents of unsaturated fatty acid and specific pinolenic acid reached 85.55% and 13.65%, respectively. The antioxidant activity and physicochemical properties of P.koraiensis seed oil were also determined. The seed oil exhibited better DPPH radical scavenging activity with the IC50 value of 0.0954 g·mL-1. And the seed oil was a high quality natural oil with lower acid value, lower peroxide value and high iodine value.

Key words: Pinus koraiensis Sieb seed oil, ultra turrax-microwave assisted extraction, unsaturated fatty acid, quality evaluation

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