Extraction Process Optimization of Inulin from Helianthus tuberosus L. by Response Surface Methodology

doi:10.7525/j.issn.1673-5102.2016.04.020

Abstract

Abstract: Single-factor tests and response surface method (RSM) were used to optimize the extraction process of Helianthus tuberosus L. inulin in tubers.In order to obtain the maximum of extraction yield of the inulin, three main factors including liquid-solid ratio, extraction times and extraction temperature were selected with the Design Expert V8.0 design, and a quadric regression equation for predicting the yield of inulin was established. The results showed that the optimum extraction conditions were as follows:liquid-solid ratio of 18:1(mL·g^-1), extraction time of 41 min and extraction temperature of 85℃. Under the conditions, the optimum extracting yield of inulin was 40.56%, which is well matched with the predictive extracting yield of 40.74%. Under the process, we carried out the rough inulin and analyzed extraction of the inulin by infrared spectrum with the simple and low cost process.

Key words: Helianthus tuberosus L., inulin, response surface methodology, extraction process

CLC Number:

TS244+.1

LIU Yan, CHEN Xiao-Yin, YANG Li-Li, XIE Rui, HE Nan, ZHANG Ji. Extraction Process Optimization of Inulin from Helianthus tuberosus L. by Response Surface Methodology[J]. Bulletin of Botanical Research, 2016, 36(4): 627-633.

References

1.孔涛,张楠,林凤梅,等.不同品种菊芋块茎及茎叶营养成分分析比较[J].广东农业科,2013,40(6):108-109,113.
Kong T,Zhang N,Liu F M,et al.Analysis and comparison of stem leaf and tubernutritionalcomposition in different Jerusalem artichoke[J].Guangdong Agricultural Sciences,2013,40(6):108-109,113.
2.Sarchami T,Rehmann L.Optimizing enzymatic hydrolysis of inulin from Jerusalem artichoke tubers for fermentative butanol production[J].Biomassand Bioenergy,2014,69:175-182.
3.孙纪录,贾英民,桑亚新.菊芋资源的开发利用[J].食品科技,2003,18(1):27-29.
Sun J L,Jia Y M,Sang Y X.The development and utilization of Jerusalem artichoke resource[J].Food Science and Technology,2003,18(1):27-29.
4.曾小宇,罗登林,刘胜男,等.菊糖的研究现状与开发前景[J].中国食品添加剂,2010(4):222-227.
Zeng X Y,Luo D L,Liu S N,et al.Recent advance and future trend ofinulin research and development[J].China Food Additives,2010(4):222-227.
5.巩慧玲,李善家,李志忠.不同品种菊芋总酚含量、PPO活性、POD活性及其与褐变的关系[J].中国蔬菜,2011,31(12):65-69.
Gong H L,Li S J,Li Z Z.Studies on relation between phenol content,activities of PPO and POD,and browning of different artichok cultivars[J].China Vegetables,2011,31(12):65-69.
6.张泽生,聂洪霞,王婧宜,等.菊芋为原料生产高果糖浆工艺技术的研究[J].食品工业,2011(6):61-63.
Zhang Z S,Nie H X,Wang J Y,et al.Study on production of high-fructosesyrup from Jerusalem artichokes[J].The Food Industry,2011(6):61-63.
7.Matías J,Encinar J M,González J,et al.Optimisation of ethanol fermentation of Jerusalem artichoke tuber juice using simple technology for a decentralisedand sustainable ethanol production[J].Energy for Sustainable Development,2015,25:34-39.
8.Li W C,Zhang J,Yu C W,et al.Extraction,degree of polymerization determination and prebioticeffect evaluation of inulin from Jerusalem artichoke[J].Carbohydrate Polymers,2015,121:315-319.
9.Kim S,Kim C H.Evaluation of whole Jerusalem artichoke(Helianthus tuberosus L.) for consolidated bioprocessing ethanol production[J].Renewable Energy,2014,65:83-91.
10.冯学花,张国升,陶阿丽,等.响应面法优化厚朴中和厚朴酚与厚朴酚的提取工艺研究[J].植物研究,2014,34(1):103-107,113.
Feng X H,Zhang G S,Tao A L,et al.Extraction of magnolol and honokiolin magnoliae cortex by response surface method[J].Bulletin of Botanical Research,2014,34(1):103-107,113.
11.朱媛,张雪松,黄小忠.响应面法优化茶多酚乙酰化工艺条件[J].天然产物研究与开发,2015,27(2):306-313.
Zhu Y,Zhang X S,Huang X Z.Optimization of acetylationconditions oftea polyphenols using response surface methodology[J].Natural Product Research and Development,2015,27(2):306-313.
12.王洪政,李媛媛,刘伟,等.响应面法优化牡丹果荚多糖提取工艺及其抗氧化活性评估[J].植物研究,2015,35(1):127-132.
Wang H Z,Li Y Y,Liu W,et al.Optimizing polysaccharide extraction from peony tree pod using response surface method and assessing its antioxidative activity[J].Bulletin of Botanical Research,2015,35(1):127-132.
13.郁玮,杨小明,刘伟民,等.苯酚-硫酸法测定无花果中多糖含量的研究[J].食品科技,2009,34(10):256-258,262.
Yu W,Yang X M,Liu W M.Assay study on content of polysaccharides in Ficus carica by phenol-sulfuric acid method[J].Food Science and Technology,2009,34(10):256-258,262.
14.樊晓辉,赵春玲,张成军,等.3,5-二硝基水杨酸法测定枸杞提取液中多糖的含量[J].食品研究与开发,2014,35(21):77-80.
Fan X H,Zhao C L,Zhang C J,et al.Determination of polysaccharide content in extraction solution of Lycrium barbarum with 3,5-dinitrosalicylicacidmethod[J].Food Research and Development,2014,35(21):77-80.
15.杨贵明,蒋爱华,薛秋生.用DNS光度法测定还原糖的条件研究[J].安徽农业科学,2006,34(14):3258,3264.
Yang G M,Jiang A H,Xue Q S.Study on the determination factorof reducedsugar with DNS spectrophotometry[J].Journal of Anhui Agricultural Sciences,2006,34(14):3258,3264.
16.赵琳静,李洪森,陈婷婷,等.菊芋菊糖含量的测定[J].化工生产与技术,2010,17(4):43-45.
Zhao L J,Li H S,Chen T T,et al.Determination of inulin from Jerusalem artichoke tubers[J].Chemical Production and Technology,2010,17(4):43-45.
17.陈秀枝,沈辰婷,曹未音,等.菊芋中菊糖提取方法的比较[J].江苏农业科学,2013,41(11):312-314.
Chen X Z,Shen C T,Cao W Y,et al.Comparative study on extraction methods of inulin from Jerusalem artichoke[J].Jiangsu Agricultural Sciences,2013,41(11):312-314.
18.林平,张莹,祖元刚,等.核桃楸叶片中总黄酮的最佳提取工艺研究[J].植物研究,2007,27(5):616-618.
Lin P,Zhang Y,Zu Y G,et al.Optimum extraction process of total flavonoids in Juglans mandshurica leaves[J].Bulletin of Botanical Research,2007,27(5):616-618.
19.许福超,薛志欣,叶乃好,等.浒苔多糖的提取、提纯和分析[J].科学技术与工程,2010,10(10):2413-2415,2433.
Xu F C,Xue Z X,Ye N H,et al.Isolation,purification and analysis on the polysaccharide of Enteromorpha[J].Science Technology and Engineering,2010,10(10):2413-2415,2433.
20.王硕,袁洪福,宋春风,等.小西瓜糖度表征与漫反射近红外检测方法的研究[J].光谱学与光谱分析,2012,32(8):2122-2125.
Wang S,Yuan H F,Song C F,et al.Sugar characterization of mini-watermelon and rapid sugar determination by near infrared diffuse reflectance spectroscopy[J].Spectroscopy and Spectral Analysis,2012,32(8):2122-2125.

[1]	Yu LI, Xiao YANG, Xiao YE, Jie-Qiong DENG, Wei-Nian HUANG, Sha DAI. Optimization on the Main Active Components Extraction Using Ultrasonic-assisted Method from Safflower [J]. Bulletin of Botanical Research, 2021, 41(5): 824-835.
[2]	ZHANG Xiao-Nan, ZHU Hong-Wei, ZHAO Shan-Bo, BAI Chen, SHI Ya-Nan, XU Hao-Peng, YIN Lian, HU Shu-Min, YIN Hua. Optimization of Ultrasound-assisted Extraction of Dihydromyricetin from Seeds of Hovenia dulcis Thunb. Using Response Surface Methodology [J]. Bulletin of Botanical Research, 2020, 40(5): 775-781.
[3]	ZHU Ya-Wei, WANG Li-Tao, AN Juan-Yan, Lü Mu-Jie, SUN Jian-Hang, GUO Na, FU Yu-Jie. Response Surface Optimized Enzymatic Hydrolysis-microwave Assisted Extraction Process of Mulberry Leaf Polysaccharide [J]. Bulletin of Botanical Research, 2020, 40(4): 635-640.
[4]	LI Wen-Gang, SUN Xiao-Li, ZU Yuan-Gang, ZHAO Xiu-Hua. Optimization Peony Seed Oil Extraction Process at Suitable Temperature and Physicochemical Property Analysis [J]. Bulletin of Botanical Research, 2020, 40(1): 73-78.
[5]	CHEN Qing1；JIANG Lei-Yu1；WANG Yan1；ZHU Fang-Li1；TANG Hao-Ru1*；WANG Xiao-Rong1,2. Optimization of Prokaryotic Expression Factors for Blackberry UGT78H2 Using Responsive Surface Methodology and Protein Purification [J]. Bulletin of Botanical Research, 2015, 35(5): 724-729.
[6]	WANG Qiu-Xue;GU Cheng-Bo;*;FU Li-Nan;LI Wang;YUAN Xiao-Han;ZU Yuan-Gang;FU Yu-Jie;. Optimization of Cellulase-assisted Extraction Process of Total Flavonoids from Pigeon Pea Leaves Using Response Surface Methodology [J]. Bulletin of Botanical Research, 2012, 32(6): 765-769.
[7]	ZHANG Yu;LIU Ting-Ting;LIU Yang;YANG Lei;ZU Yuan-Gang. Extraction Process and Antioxidant Activity of Polyphenols from Testa of Quercus mongolica* Fischer [J]. Bulletin of Botanical Research, 2010, 30(5): 623-628.
[8]	LIN Ping;ZHANG Ying;ZU Yuan-Gang;WANG Wen-Jie. Optimum Extraction Process of Total Flavonoids in Juglans mandshurica* Leaves [J]. Bulletin of Botanical Research, 2007, 27(5): 616-618.