Optimization on Extractive Technique of Phenylalanine Ammonia-lyase in Lily Bulb

doi:10.7525/j.issn.1673-5102.2008.05.019

Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (5): 594-598.doi: 10.7525/j.issn.1673-5102.2008.05.019

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Optimization on Extractive Technique of Phenylalanine Ammonia-lyase in Lily Bulb

SUN Hong-Mei;ZHAO Shuang;WANG Chun-Xia;CHEN Li-Jing;WANG Jin-Xia

(College of Horticulture,Shenyang Agricultural University,Key Laboratory of Protected Horticulture of Liaoning Province,Shenyang　110161)

Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20
Contact: SUN Hong-Mei
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Abstract

Abstract: Optimum extractive technique of phenylalanine ammonia-lyase(PAL) in the bulb of Lilium davidii var. unicolor was studied. The results indicated that Borax solution with pH8.8 was the best buffer. PAL in lily bulb was not able to endure the strong acid and alkali, especially the former. The PAL activity declined gradually along with the increase of temperature and prolonging of bath duration, but the activity still remained 47% after 30 min in 40℃, and it was the optimum bath temperature. 0.032 mol·L^-1 substrate was the best, and grinding with 0.5 g PVP could improve the PAL activity obviously.

Key words: Lilium davidii var. unicolor, Bulb, Phenylalanine ammonia-lyase(PAL), extractive technique

CLC Number:

SUN Hong-Mei;ZHAO Shuang;WANG Chun-Xia;CHEN Li-Jing;WANG Jin-Xia. Optimization on Extractive Technique of Phenylalanine Ammonia-lyase in Lily Bulb[J]. Bulletin of Botanical Research, 2008, 28(5): 594-598.

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Optimization on Extractive Technique of Phenylalanine Ammonia-lyase in Lily Bulb

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