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Bulletin of Botanical Research ›› 2007, Vol. 27 ›› Issue (6): 736-740.doi: 10.7525/j.issn.1673-5102.2007.06.016

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AFLP Analysis of Genetic Diversity Among Lespedeza bicolor Populations

WANG Xiu-Rong;ZHAO Yang;*;CHEN Xiao-Yang;PIAN Rui-Qi   

  1. 1.College of Forest,Guizhou University,Guiyang　550225 2.Beijing Forestry University,Beijing　100083
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-11-20 Published:2007-11-20
  • Contact: ZHAO Yang
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Abstract: Genetic diversities of Lespedeza bicolor were evaluated using AFLP in this study. Five pairs of primers were used for evaluating genetic diversity. 253 AFLP markers were observed,among which 245 markers (PPL=96.8%) were polymorphic. At species level,Ao=1.968,Ae=1.643,H=0.369 and I=0.544. And at population level,P=62.99%,Ao=1.605,Ae=1.479,H=0.257,I=0.373. The coefficient of genetic differentiation was 0.233 2, which indicated that 76.68% variability occurred in populations of L.bicolor 14 populations of L.bicolor were divided into three groups by the dendrogram constructed using Nei’s genetic distance. There was no relativity between the genetic diversity parameters (P,H,I) and geographic & climatic factors (longitude,latitude,AMT,AMP), and no fixed distribution pattern of genetic diversity was found either.

Key words: Lespedeza bicolor, AFLP, genetic diversity

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