Abstract: Genomic DNA was extracted from leaves of Vitis amurensis, Actinidia arguta, Quercus mongolica, Juglans mandshurica and needles of Pinus sibirica, Pinus pumila by the method of modified CTAB. The isolated genomic DNA length was similar to λDNA (48 kb) and is suitable for both PCR amplification and digestion with restriction endonucleases. The absorbance ration (A₂₆₀/A₂₈₀) ranged 1.66～1.89. The quality of DNA extracted from third and fourth times suspension was better than from first and second times suspension. The yields of DNA ranged from 15 μg·g^-1 fresh mass（from fourth time suspension J.mandshuric） to 272 μg·g^-1 fresh mass（from third time suspension of V.amurensis）.Although from first and second times suspension of P.pumila and P.sibirica wasn’t isolated genomic DNA, but from third and fourth times suspension was obtained high quality genomic DNA. The genomic DNA can be used for next analysis via identification of PCR and digestion with restriction endonucleases.

Key words: woody plants, genomic DNA, extraction