DNA EXTRACTION AND RAPD ANALYSISOF SARGENTODOXA CUNEATA

Abstract

Abstract: DNA extraction and RAPD amplification of Sargentodoxa cuneata was studied. The results showed that the good quality DNA of Sargentodoxa cuneata was gained using improved SDS method and the length of DNA fragment was more than 23kb. The isolatd DNA could be used for RAPD analyses with more purification. RAPD markers were examined in 12 individuals Sargentodoxa cuneata, and 14 of 15 primers reveal polymorphisms. The RAPD analysis procedure in this experiment lays a good foundation for application of RAPD in Sargentodoxa cuneata genetic research.

Key words: Sargentodoxa cuneata, DNA, extraction, RAPD

LI Jun-min, JIN Ze-xin, BIAN Cai-miao, ZHONG Zhang-cheng. DNA EXTRACTION AND RAPD ANALYSISOF SARGENTODOXA CUNEATA[J]. Bulletin of Botanical Research, 2002, 22(4): 483-486.

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DNA EXTRACTION AND RAPD ANALYSISOF SARGENTODOXA CUNEATA

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