Bulletin of Botanical Research ›› 2002, Vol. 22 ›› Issue (4): 487-491.
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GUO Dong-lin, WANG Tong-cang, WANG Ning, XU Shu-hong, LI Ji-lin
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Abstract: Objective Gene Rf3 originated from chromosome 1B of Triticum spelta var. Duhameliancum is a fertility-restorer gene for G-type cytoplasmic male sterility. Microdissected chromosome 1B by glass needles from cells in duration of meiotic metaphase. The DNA fragments was amplified by means of LA-PCR. By dot hybridization, the product was confirmed as the amplified DNA from chromosome 1B of T. spelta var. duhameliancum.
Key words: microdissection, G-type cytoplasmic male sterlity, gene Rf3, Chromosome1B, LA-PCR
GUO Dong-lin, WANG Tong-cang, WANG Ning, XU Shu-hong, LI Ji-lin. MICRODISSECTION OF CHROMOSOME 1B OF TRITICUM SPELTA VAR. DUHAMELIANCUM AND AMPLIFICATION OF THE CHROMOSOME DNA[J]. Bulletin of Botanical Research, 2002, 22(4): 487-491.
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https://bbr.nefu.edu.cn/EN/Y2002/V22/I4/487
