Abstract: The tissue culture of Regensberg with the tip of shoots as the explants was experimnented in MS medium with addition of different contentration and kinds of multiplication.The results show that sprouting of bud was best on MS medium with addition of 6-BA 1.0mg/l;and the multiplication of adventitious buds was best on MS medium with the addition of 6-BAl.o+NAA0.05mg/l;and vigorous and healthy bud was best on MS mdeuim with addition of KT0.3+NAA 0.05+ZT0.1mg/lor6-BA0.3+NAA0.05+ZT0.1mg/l;and the growth of roots was best on 1/2 MS medium with the addition IBA0.5+NAA0.3mg/lor IAA0.5+NAA0.3mg/l.It was better to transplant from test-tube grew to 2-3 cm high and 4-8roots and after the plantlets stayed 3 or 5 days without cap of test-tube in the experimental. The plantlets had better percentage of survival when it had the situation with deeping degree of temperature and humidity.

Key words: Regensberg, Cytokinin, Auxin