Construction and Transformation of Arabidopsis CKX3 Gene Editing Vector Based on CRISPR/Cas9

doi:10.7525/j.issn.1673-5102.2021.06.020

Abstract

CLC Number:

Q789

Ze-Chen WANG, Rong XIAO, Le-Jun OUYANG, Li-Mei LI, Chu-Yan LIANG, Jing-Yin PAN, Zhi-Chao LIU. Construction and Transformation of Arabidopsis CKX3 Gene Editing Vector Based on CRISPR/Cas9[J]. Bulletin of Botanical Research, 2021, 41(6): 1015-1022.

Figures/Tables 8

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References 17

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引物名称 Primer name	引物序列 Primer sequence（5′→3′）	引物用途 Primers use
CKX3-F	ctagagtcgaagtagtgattgTCCACCGTCCGATCACTTGgttttagagctagaaatagc	载体构建 Vector construction
CKX3-R	tgctatttctagctctaaaacCGGGTATAGCGGCAGACATCaatctcttagtcgactcta	载体构建 Vector construction
CKX3-GT1	CTTCGCGGTGTTAGGAGGTT	鉴定引物 Identify primers
CKX3-GT2	TCCACCTCGGACCAAAATGT	鉴定引物 Identify primers

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Construction and Transformation of Arabidopsis CKX3 Gene Editing Vector Based on CRISPR/Cas9

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