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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (1): 148-154.doi: 10.7525/j.issn.1673-5102.2018.01.018

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Arabidopsis thaliana Transcription Factor Ethylene-insensitive3(EIN3) Inhibits the Synthesis of Anthocyanins

XU Meng-Ke1,2, LI Dan1,2, MENG Lai-Sheng1,2, JIANG Ji-Hong1,2   

  1. 1. The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou 221116;
    2. College of Life Science, Jiangsu Normal University, Xuzhou 221116
  • Received:2017-05-11 Online:2018-01-15 Published:2018-01-06
  • Supported by:
    Agricultural Science and Technology(Agricultural High-tech Project)(KC16NG063)

Abstract: Ethylene-insensitive3(EIN3) and EIN3-like1(EIL1) proteins are important nuclear transcription factors in ethylene signal transduction pathways in higher plants. In plants, anthocyanins are one of the water-soluble natural pigments, and they play an important role in the growth and development of plants. We used the Arabidopsis thaliana double mutant ein3-1eil1-3 as the research material. Firstly, we identified the mutants of ein3-1eil1-3 and ein3-1 by RT-PCR. The direct observation indicated that the seeds and leaves of the ein3-1eil1-3 were purple. By ultraviolet spectrophotometer analysis, the anthocyanin accumulation in ein3-1eil1-3 mutant was significantly higher than that in the ein3-1 mutant and wild-type. GUS staining showed that EIN3 was mainly expressed in flowers, stigma, mature pollen, seed embryo and siliques, which was consistent with that anthocyanin accumulation of seeds and leaves was high in mutant ein3-1eil1-3. Thus, A.thaliana transcription factor EIN3 as well as EIL1 is involved in the suppression of the anthocyanin synthesis.

Key words: ethylene-insensitive3(EIN3), ethylene-insensitive3-like 1(EIL1), anthocyanins, Arabidopsis thaliana

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