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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (1): 88-95.doi: 10.7525/j.issn.1673-5102.2017.01.012

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Assessment of Genetic Diversity of Rhododendron nivale in Tibetan, China using AFLP Markers

XU Jing-Jing1, ZHAO Bing1, ZHANG Liang-Ying2, SHEN Hui-Fei1, LI Hou-Hua1   

  1. 1. The College of Landscape Architecture and Arts, Northwest A & F University, Yangling 712100;
    2. College of agriculture and animal husbandry, Tibet University, Linzhi 860114
  • Received:2016-08-30 Online:2017-01-15 Published:2017-03-06
  • Supported by:
    National Science Foundation(K305021401)

Abstract: To determine the correlation among genetic variations, the geographic location of a population, and factors that influence high-level genetic diversity, the genetic diversity of 135 Rhododendron nivale samples was studied using amplified fragment length polymorphisms(AFLP). A total of 273 amplification products were generated from 6 selective primer combinations, of which 85.71% were polymorphic. The results of POPGENE showed the change trends of the percentage of polymorphic(PPL), Nei's genetic diversity(h) and Shannon's index(I) were similar. The change trends of Nei's genetic diversity(h) and Shannon's index(I) were Gongbujiada < Milin < Galongla < Sejila Mountain < Hongla Mountain. The POPGENE analysis showed that R.nivale had higher genetic diversity at species level(PPL=85.71%, I=0.415 1, h=0.273) but lower at population level(PPL=62.26%, I=0.280 3, h=0.184 1). Both the results of POPGENE(Gst=0.324) and AMOVA(36% among populations, 64% within populations) showed that the most genetic variance existed within the populations. UPGMA tree plots indicated that there was no significant correlation between the genetic distance and the geographic distance among the R.nivale populations. At last, some conservation strategies for R.nivale germplasm were put forward.

Key words: Tibetan, Rhododendron nivale, genetic diversity, AFLP, population

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