Genetic Diversity and Fingerprints with SCoT Markers in Actinidia

doi:10.7525/j.issn.1673-5102.2017.02.014

Abstract

Abstract: SCoT were used to analysis genetic diversity of the samples of 32 Actinidia varieties. The 11 primers were screened from 47 SCoT primers and 185 SCoT bands were obtained, including 180 polymorphic bands, with a polymorphism rate of 97.30%. The average Nei's gene diversity(H) and Shannon's information index(I) were 0.2384 and 0.3778. The cluster analysis conducted with UPGMA showed that 32 Actinidia varieties were divided into 5 groups at the genetic similarity coefficient of 0.78, and the constructed phylogenetic trees based on SCoT were consistent with the morphology results. The DNA fingerprints for 32 Actinidia varieties were established with 16 sites from 4 SCoT primers, and 32 Actinidia varieties could be identified by SCoT fringerprints.

Key words: Actinidia, SCoT, DNA fingerprint, genetic diversity

CLC Number:

S663.4

ZHANG An-Shi, ZHANG Zhong-Hai, QI Xiu-Juan, LIU Ying, LUO Yang. Genetic Diversity and Fingerprints with SCoT Markers in Actinidia[J]. Bulletin of Botanical Research, 2017, 37(2): 259-265.

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