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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (5): 562-567.doi: 10.7525/j.issn.1673-5102.2010.05.008

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Medium Optimization for Callus Proliferation of Bambusa multiplex

YUAN Jin-Ling;YUE Jin-Jun;WU Xiao-Li;ZHANG Duo;GUO Guang-Ping;GU Xiao-Ping*   

  1. Research Institute of Subtropical Forestry,CAF,Fuyang 311400
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
  • Contact: GU Xiao-Ping
  • Supported by:

Abstract: An effective regeneration system is the pre-requisite for genetic transformation of bamboos. Medium components for callus proliferation of Bambusa multiplex were studied, 5 basal medium, 6 organic additives, 7 sugars, and 5 macronutrients were compared. The results showed that Murashige and Skoog (MS) basal medium was effective, callus increased 2.8 times at 20 days, white to yellowish, compact; 1.0 g·L-1 proline was suitable the organic additive, callus increased 3.64 times, yellowish, compact and even; 30 g·L-1 maltose was the optimal carbon source, callus increased 2.96 times, white to yellowish, even. Among five macronutrients, NH4NO3 has significant influence on callus proliferation (α=0.05) and its optimal concentration was 825 mg·L-1, callus could increase over 5 times at 29 days, with some rooting; KNO3 475 mg·L-1+NH4NO3 825 mg·L-1+MgSO4·7H2O 185 mg·L-1+KH2PO4 340 mg·L-1+CaCl2·7H2O 440 mg·L-1 is a suitable combination for callus proliferation.

Key words: Bambusa multiplex, callus, proliferation, medium, optimization

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