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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (1): 153-160.doi: 10.7525/j.issn.1673-5102.2020.01.021

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Rapid Propagation of Virus-free “Dan Mei 1” Strawberry Using Tissue Culture Technology

WANG Xin, GAI Qing-Yan, JIAO Jiao, FU Yu-Jie, LIU Jing, WANG Zi-Ying   

  1. Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040
  • Received:2019-03-06 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572017DA04,2572018BU02)

Abstract: The superior "Dan Mei 1" strawberry variety that is suitable for growing in Northeast China were selected as the research object, and the tissue culture technology for detoxification and rapid propagation of strawberry was carried out systematically. The effects of different plant hormones on adventitious bud induction, proliferation and rootage were investigated using the stem tip of "Dan Mei 1" strawberry as explants. Also, the effects of transplanting medium on the domestication survival of strawberry plantlets were investigated. The results showed that MS with 1 mg·L-1 6-BA+0.1 mg·L-1 2,4-D was suitable for adventitious bud induction. The best medium for adventitious bud proliferation was MS with 1 mg·L-1 6-BA+0.3 mg·L-1 NAA. And, MS with 0.4 mg·L-1 IBA was the best medium for adventitious bud rootage. Perlite:vermiculite:nursery soil with the ratio of 1:2:1 was the best transplanting medium for the domestication of strawberry plantlets. Overall, this study provided a theoretical foundation for the large-scale propagation of the virus-free "Dan Mei 1" strawberry variety.

Key words: “Dan Mei 1” strawberry, stem tip differentiation, rapid proliferation, virus-free plantlets

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