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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (6): 692-695.doi: 10.7525/j.issn.1673-5102.2009.06.009

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Cloning and Prokaryotic Expression of Chorispora bungeana CbMAPK3 Gene

ZHANG Teng-Guo;LIU Yu-Bing;SUN Kun;YANG Ning;AN Li-Zhe*   

  1. (1.School of life science,Northwest Normal University,Lanzhou 730070) (2.Cold and Arid Regions Environmental and Engineering Institute,Chinese Academy of Sciences,Lanzhou 730000) (3.College of Life Science,Lanzhou University,Lanzhou 730070)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
  • Contact: AN Li-Zhe
  • Supported by:

Abstract: Mitogen-activated protein kinase (MAPK) plays a central role in transfer information from diverse receptors/sensors to a wide range of cellular responses in plants. CbMAPK3 cDNA was amplified by RT-PCR from Chorispora bungeana leaf, then was cloned into pET-30a vector to construct recombinant prokaryotic expression vector pET-30a-CbMAPK3. The pET-30a-CbMAPK3 was transformed to E.coli strain of BL21. After induced by IPTG, a 46 kD recombinant protein was expressed and separated by SDS-PAGE electrophoresis and detected by western blot. The research provides a base for further studying on the protein structure and function of CbMAPK3.

Key words: Chorispora bungeana, CbMAPK3 gene, prokaryotic expression

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