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植物研究 ›› 2009, Vol. 29 ›› Issue (2): 242-244.doi: 10.7525/j.issn.1673-5102.2009.02.020

• 论文 • 上一篇    下一篇

一种禾本科植物鲜叶下表皮制片方法

杨虎彪;李晓霞;罗丽娟*   

  1. (海南大学园艺园林学院,儋州 571737)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-20 发布日期:2009-03-20
  • 通讯作者: 罗丽娟
  • 基金资助:
     

A Method for Lower Epidermis Micromorphology Research of Fresh Leaf of Gramineae

YANG Hu-Biao;LI Xiao-Xia;LUO Li-Juan*   

  1. (College of Horticulture & Garden architecture,University of Hainan,Danzhou 571737)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: LUO Li-Juan*
  • Supported by:
     

摘要: 介绍一种禾本科植物鲜叶下表皮制片的快速方法,该方法也能适用于多数单子叶植物鲜叶和经FAA固定液固定的单子叶植物叶片的气孔观察。该方法制片能有效防止叶表皮脱水、透明后发生卷曲,使保持完整的结构,便于准确的显微观察。具操作简单、材料易得、可靠性和稳定性高等优点。

关键词: 禾本科, 鲜叶, 下表皮, 方法

Abstract: The aim of this study was to introduce an efficiect and fast method for observation the lower epidermis of fresh leaf of Gramineae, it also can be used for observation the stomata of Monocotyledon whatever fresh leaf or have been fixed in FAA solution. This method can avoid crispation when epidermis dehydration in 100% ethanol and clearing in Xylene to keep the full framework for accurate and efficient observation of microstructure. Furthermore, this method exhibits higher reliability and stability for micromorphology research of leaf epidermis of Gramineae and Monocotyledon.

Key words: Gramineae, fresh leaf, lower epidermis, method

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