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植物研究 ›› 2021, Vol. 41 ›› Issue (1): 98-106.doi: 10.7525/j.issn.1673-5102.2021.01.013

• 研究报告 • 上一篇    下一篇

橡胶草E2泛素结合酶基因TkUBC2基因的克隆及其表达分析

王肖肖1,2, 覃碧2, 杨玉双2, 聂秋海3, 张继川4, 刘实忠2()   

  1. 1.海南大学热带作物学院,海口 570228
    2.中国热带农业科学院橡胶研究所,海口 571101
    3.北京玲珑蒲公英科技发展有限公司,北京 110000
    4.北京化工大学材料科学与工程学院,北京 100029
  • 收稿日期:2020-07-02 出版日期:2021-01-20 发布日期:2021-01-05
  • 通讯作者: 刘实忠 E-mail:sz_liu@126.com
  • 作者简介:王肖肖(1996—),女,硕士研究生,主要从事农艺与种业研究。
  • 基金资助:
    国家重点研发专项(2017YFB0306901);北京玲珑蒲公英科技发展有限公司研发课题(橡胶草种质收集、创新与良种繁育);中国热带农业科学院橡胶研究所基本科研业务费专项(1630022019015)

Cloning and Expression Analysis of E2 Ubiquitin-conjugating Enzyme Gene TkUBC2 in Taraxacum kok-saghyz Rodin

Xiao-Xiao WANG1,2, Bi QIN2, Yu-Shuang YANG2, Qiu-Hai NIE3, Ji-Chuan ZHANG4, Shi-Zhong LIU2()   

  1. 1.College of Tropical Crops,Hainan University,Haikou 570228
    2.Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
    3.Beijing Linglong Dandelion Technology and Development Ltd,Beijing 110000
    4.College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029
  • Received:2020-07-02 Online:2021-01-20 Published:2021-01-05
  • Contact: Shi-Zhong LIU E-mail:sz_liu@126.com
  • About author:WANG Xiao-Xiao(1996—),female,master,mainly engaged in the agronomy and seed industry.
  • Supported by:
    National Key Research and Development Project(2017YFB0306901);Research and Development Project of Beijing Linglong Dandelion Technology and Development Ltd.(Germplasm collection, innovation and breeding of Taraxacum kok-saghyz Rodin);Earmarked Funds from Rubber Research Institute of Chinese Academy of Tropical Agricultural Sciences(1630022019015)

摘要:

为解析橡胶草E2泛素结合酶在胁迫响应和信号转导中的功能,本文从橡胶草品系1151中克隆得到一个E2泛素结合酶基因,命名为TkUBC2该基因编码区cDNA为459 bp,编码152个氨基酸。序列比对分析发现,不同物种间的UBC2高度同源,TkUBC2与莴苣、向日葵的UBC2相似性高达99%以上。采用荧光定量qRT-PCR技术分析该基因在橡胶草中的表达模式,结果表明,TkUBC2在不同组织中均有表达;而且PEG6000模拟干旱胁迫、甘露醇介导的渗透压胁迫以及植物激素茉莉酸甲酯(MeJA)、脱落酸(ABA)和乙烯利(ET)可诱导TkUBC2下调表达;而NaCl盐胁迫和UV辐射处理则上调TkUBC2表达。结果表明TkUBC2参与橡胶草抗逆反应、激素信号转导以及DNA损伤修复过程,以上结果为进一步解析该基因的功能奠定良好的基础。

关键词: 橡胶草, TkUBC2, 非生物胁迫, 表达分析

Abstract:

In order to analyze the function of E2 ubiquitin-conjugating enzyme(UBC) in stress response and signal transduction, an UBC gene was cloned from Taraxacum kok-saghyz Rodin(TKS) line 1151 and named as TkUBC2. The ORF of this gene was 459 bp and encoded 152 aa. Sequence alignment analysis showed that UBC2 shared high level homology among different species, and TkUBC2 had more than 99% similarity with Lactuca sativa LsUBC2 and Helianthus annuus HaUBC2. qRT-PCR was used to analyze the expression pattern of TkUBC2in TKS. The results indicated that TkUBC2 was ubiquitously expressed in different tissues. Moreover, TkUBC2expression was down-regulated by treatments of PEG6000 simulated drought stress, mannitol mediated osmotic stress and plant hormone i.e. methyl jasmonate(MeJA), abscisic acid(ABA) and ethylene(ET) respectively, but its expression was up-regulated by the treatments of NaCl salt stress and UV radiation respectively. The results of this study suggested that TkUBC2 was involved in response to various stress conditions, hormone signaling and DNA damage repair process, which lay a good foundation for further revealing the function of TkUBC2 in TKS.

Key words: Taraxacum kok-saghyz Rodin, TkUBC2, abiotic stress, expression analysis

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