小黑杨PsnHB22基因在烟草中的遗传转化研究

doi:10.7525/j.issn.1673-5102.2020.05.015

植物研究 ›› 2020, Vol. 40 ›› Issue (5): 760-767.doi: 10.7525/j.issn.1673-5102.2020.05.015

小黑杨PsnHB22基因在烟草中的遗传转化研究

李潺¹, 张翔³, 郑兰², 常成龙², 刘彩霞³, 郑密³, 由香玲¹

1. 东北林业大学生命科学学院, 哈尔滨 150040;
2. 四平市国有林总场石岭子落叶松国家良种基地, 四平 136000;
3. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2020-01-02 出版日期:2020-09-05 发布日期:2020-07-10
通讯作者: 由香玲,E-mail:yxiangling@yahoo.com E-mail:yxiangling@yahoo.com
作者简介:李潺(1995-),女,硕士研究生,主要从事植物基因工程方面的研究。
基金资助:
国家自然科学基金项目（31700596）

Genetic Transformation of PsnHB22 Gene from Populus simonii×P.nigra in Tobacco

LI Chan¹, ZHANG Xiang³, ZHENG Lan², CHANG Cheng-Long², LIU Cai-Xia³, ZHENG Mi³, YOU Xiang-Ling¹

1. College of Life Sciences, Northeast Forestry University, Harbin 150040;
2. Siping State-owned Forest Farm Shilingzi Larch National Improved Seed Base, Siping 136000;
3. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2020-01-02 Online:2020-09-05 Published:2020-07-10
Supported by:
National Natural Science Foundation of China(31700596)

摘要/Abstract

摘要： HD-Zip转录因子在光信号转导、非生物胁迫、叶片发育等方面发挥重要的作用，HB22转录因子是HD-ZipⅠ亚家族的成员之一。为研究PsnHB22基因的功能，从小黑杨（Populus simonii×P.nigra）cDNA中克隆PsnHB22基因并构建植物表达载体进行烟草（Nicotiana tabacum）的遗传转化，以获得该基因过量表达的转基因株系。对转基因株系进行PCR、qRT-PCR分子检测后观察表型，结果显示在营养生长时期，转基因烟草叶片窄小并且株高显著低于野生型对照。测定转基因烟草及野生型叶片的叶绿素含量，发现转基因烟草叶绿素含量显著高于野生型。由此推测PsnHB22基因在植株高生长、光合作用及叶片的形态建成等过程中起着重要的作用。

关键词: 小黑杨, PsnHB22基因, HD-Zip转录因子, 烟草, 叶绿素含量

Abstract: HD-Zip gene family plays an important role in light signal transduction, abiotic stress and leaf development. HB22 transcription factor is a member of HD-ZipⅠ subfamily. In order to investigate the function of PsnHB22 gene, PsnHB22 gene was cloned from Populus simonii×P.nigra. The expression vector was transformed into tobacco(Nicotiana tabacum). The phenotype of transgenic tobacco was observed after PCR and qRT-PCR detection. The results showed that the leaves of transgenic tobacco were narrow and the plants were significantly lower than those of wild type tobacco during vegetative growth period. The chlorophyll content of transgenic tobacco and wild-type tobacco leaves was determined. The chlorophyll content of transgenic tobacco was significantly higher than that of wild-type tobacco. PsnHB22 gene plays an important role in plant height growth, photosynthesis and leaf morphogenesis.

Key words: Populus simonii×P.nigra, PsnHB22 gene, HD-Zip transcription factor, Nicotiana tabacum, chlorophyll content

中图分类号:

Q943.2

李潺, 张翔, 郑兰, 常成龙, 刘彩霞, 郑密, 由香玲. 小黑杨PsnHB22基因在烟草中的遗传转化研究[J]. 植物研究, 2020, 40(5): 760-767.

LI Chan, ZHANG Xiang, ZHENG Lan, CHANG Cheng-Long, LIU Cai-Xia, ZHENG Mi, YOU Xiang-Ling. Genetic Transformation of PsnHB22 Gene from Populus simonii×P.nigra in Tobacco[J]. Bulletin of Botanical Research, 2020, 40(5): 760-767.

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小黑杨PsnHB22基因在烟草中的遗传转化研究

Genetic Transformation of PsnHB22 Gene from Populus simonii×P.nigra in Tobacco

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