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植物研究 ›› 2020, Vol. 40 ›› Issue (6): 897-905.doi: 10.7525/j.issn.1673-5102.2020.06.012

• 研究报告 • 上一篇    

转基因金叶银中杨叶色及生长变异分析

李艺迪, 顾宸瑞, 冮慧欣, 刘桂丰, 陈肃, 姜静()   

  1. 林木遗传育种国家重点实验室,东北林业大学,哈尔滨 150040
  • 收稿日期:2020-02-28 出版日期:2020-11-20 发布日期:2020-11-04
  • 通讯作者: 姜静 E-mail:jiangjing1960@126.com
  • 作者简介:李艺迪(1995—),女,硕士研究生,主要从事林木遗传育种方面的研究。
  • 基金资助:
    东北地区珍贵树种良种选育、高效培育技术示范与推广课题(GA19B201)

Leaf Color and Growth Variation of Transgenic Gold Leaf Poplar

Yi-Di LI, Chen-Rui GU, Hui-Xin GANG, Gui-Feng LIU, Su CHEN, Jing JIANG()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-02-28 Online:2020-11-20 Published:2020-11-04
  • Contact: Jing JIANG E-mail:jiangjing1960@126.com
  • About author:LI Yi-Di(1995—),female,master,main research directions:tree breeding.
  • Supported by:
    Project demonstration and promotion of precious tree species and high-quality breeding techniques in Northeast China(GA19B201)

摘要:

PaGLK基因银中杨抑制表达株系叶绿素含量显著降低,叶片呈现黄色(命名为金叶银中杨),以转PaGLK基因的银中杨为材料,测定其叶色参数和叶绿素含量的时序变化规律、分析生长特性。结果显示,转PaGLK基因的银中杨使叶片颜色发生改变,抑制表达株系整个生长期叶绿素含量显著低于WT(P<0.05),叶色亮度显著高于WT(P<0.05),并且在生长发育期叶片一直呈现深黄绿色。抑制表达株系中的Y2速生期内苗高日生长量(GD)高于对照株系,苗期株高不受影响。而过表达转基因银中杨的当年高生长都显著低于对照株系 (P<0.05),其速生期内苗高日生长量均值(GD)也低于对照株系,其均值为对照株系的22.19%。PaGLK抑制表达株系在城市园林绿化具有潜在的应用价值。

关键词: 银中杨, PaGLK, 叶绿素含量, 生长特性

Abstract:

The chlorophyll content of the PaGLK gene Populus alba×P.berolinensis inhibited expression line was significantly reduced, and the leaves showed yellow(named golden leaf silver poplar). Using PaGLK transgenic Populus alba×P.berolinensis, its leaf color parameters and chlorophyll content were measured in time series, and its growth characteristics were analyzed. The results showed that PaGLK transgenic Populus alba×P.berolinensis changed the leaf color, and the chlorophyll content of the expressing lines was significantly lower than that of WT(P<0.05), and the leaf brightness was significantly higher than that of WT(P<0.05). During the growth and development period, the leaves showed dark yellow green. The daily growth height(GD) of seedling height in the Y2 fast-growing stage of the expressing lines was higher than that of the control lines, and the plant height at the seedling stage was not affected. The over-expressing transgenic Populus alba×P.berolinensis plants were significantly lower than the control plants in that year(P<0.05), and the average daily growth height(GD) of the seedling height in the fast-growing period was also 22.19% of that of the control plants. PaGLK inhibited expression strains have potential application value in urban landscaping.

Key words: Populus alba×P. berolinensis, PaGLK, chlorophyll content, growth characteristics

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