不同地区草乌种子中生物碱含量的测定与分析

doi:10.7525/j.issn.1673-5102.2020.05.016

植物研究 ›› 2020, Vol. 40 ›› Issue (5): 768-774.doi: 10.7525/j.issn.1673-5102.2020.05.016

不同地区草乌种子中生物碱含量的测定与分析

山丹, 屈璐璐, 李晓杰, 马一鸣, 王俊杰

内蒙古农业大学草原与资源环境学院, 呼和浩特 010011

收稿日期:2019-08-16 出版日期:2020-09-05 发布日期:2020-07-10
通讯作者: 王俊杰,E-mail:jjw62@163.com E-mail:jjw62@163.com
作者简介:山丹(1995-),女,硕士研究生,主要从事药用植物资源与利用研究。
基金资助:
内蒙古自治区科技成果转化项目（CGZH2018134）

Determination and Analysis of Alkaloid Content in Aconitum kusnezoffii Seeds from Different Areas

SHAN Dan, QU Lu-Lu, LI Xiao-Jie, MA Yi-Ming, WANG Jun-Jie

College of Grassland Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010011

Received:2019-08-16 Online:2020-09-05 Published:2020-07-10
Supported by:
Scientific and Technological Achievements Transformation Project of Inner Mongolia Autonomous Region(CGZH2018134)

摘要/Abstract

摘要： 测定不同产地野生草乌（Aconitum kusnezoffii Reichb.）种子中生物碱类含量，并与草乌入药部分（块根、叶）生物碱含量做对比。采用HPLC法测定了内蒙古通辽市扎鲁特旗、赤峰市阿鲁科尔沁旗和赤峰市巴林右旗野生草乌的种子，色谱柱Supersil ODS2 5 μm（250 mm×4.6 mm，5 μm），流动相为甲醇-0.1 mol·L^-1：三乙胺（75：25），流速为0.8 mL·min^-1，检测波长为235 nm，柱温为35℃。草乌种子中检测到生物碱类，以苯甲酰新乌头原碱含量最高。通辽市扎鲁特旗种子含量为7.647 9 mg·g^-1；赤峰市阿鲁科尔沁旗种子含量为8.256 3 mg·g^-1；赤峰市巴林右旗种子含量为7.512 6 mg·g^-1，双酯型生物碱含量较低。不同产地的总乌头碱含量通辽市的高于赤峰市的，同为赤峰市含量之间显著差异。相关文献报道草乌药材（块根）中双酯型生物碱的含量明显高于单酯型生物碱，本研究实验表明，不同地区草乌种子中单酯型生物碱的含量明显高于双酯型生物碱，为草乌种子的综合利用开发及筛选不同含量种质材料提供理论依据。

关键词: 不同地区, 草乌种子, HPLC, 生物碱类, 苯甲酰新乌头原碱

Abstract: The experiment was conducted to determine the content of alkaloid in the seed of Aconitum kusnezoffii Reichb. from different habitats, and to compare its alkaloid content with its content in root and leaf. High pressure liquid chromatography(HPLC) was used to determine alkaloid content in seed of wild A.kusnezoffii collected from Zhalute of Tongliao City, Aru Kerqin of Chifeng City, and Bahrain of Chifeng City. The HPLC column used was Supersil ODS2 5 μm(250 mm×4.6 mm, 5 μm). The HPLC mobile phase was 0.1 mol·L^-1 methanol and triethylamine(V:V 75:25) and the flow rate was 0.8 mL·min^-1 under the detection wavelength of 235 nm with column temperature as 35℃. Two types of alkaloid were detected in the seed of A.kusnezoffii and the content of benzoyl aconitine was the highest while the content of diester alkaloid was comparatively lower. Total alkaloid content in seed content from Zhalute of Tongliao City, Alu Kerqin of Chifeng City, and Balin of Chifeng City was 7.647 9, 8.256 3 and 7.512 6 mg·g^-1, respectively. The total A.kusnezoffii seed alkaloid content collected from Tongliao was significantly higher than that from Chifeng City. The content of monoester alkaloid in A.kusnezoffii seed was significantly higher than diester alkaloid, and can be used for the comprehensive utilization, screening, and breeding of A.kusnezoffii germplasm with different seed alkaloid content.

Key words: different regions, Aconitum kusnezoffii seed, HPLC, alkaloid, benzoyl neoaconitine

中图分类号:

Q946.88

山丹, 屈璐璐, 李晓杰, 马一鸣, 王俊杰. 不同地区草乌种子中生物碱含量的测定与分析[J]. 植物研究, 2020, 40(5): 768-774.

SHAN Dan, QU Lu-Lu, LI Xiao-Jie, MA Yi-Ming, WANG Jun-Jie. Determination and Analysis of Alkaloid Content in Aconitum kusnezoffii Seeds from Different Areas[J]. Bulletin of Botanical Research, 2020, 40(5): 768-774.

参考文献

[1] 张跃华,贾振宇,马维文,等.草乌种子产量因子与产量间的关联性分析[J].种子,2016,35(11):87-90.Zhang Y H,Jia Z Y,Ma W W,et al.Correlation analysis between yield factors and seed yields of Aconitum kusnezoffii Reichb[J].Seed,2016,35(11):87-90.
[2] 国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010:220-221.Pharmacopoeia of the People's Republic of China 1[S].Beijing:China Pharmaceutical Science and Technology Press,2010:220-221.
[3] 国家药典委员会.中华人民共和国药典2015年版一部[S].北京:中国医药科技出版社,2015:236-237.National Pharmacopoeia Commission.Pharmacopoeia of the people's republic of China[S].Beijing:China Medical Science and Technology Press,2015:236-237.
[4] 张聿梅,谢黔锋,鲁静,等.草乌药材标准修订研究[J].药物分析杂志,2010,30(5):940-944.Zhang Y M,Xie Q F,Lu J,et al.Revision study on quality standard of Radix Aconiti kusnezoffii[J].Chinese Journal of Pharmaceutical Analysis,2010,30(5):940-944.
[5] 黄志芳,易进海,陈东安,等.制川乌HPLC特征图谱研究和6种酯型生物碱的含量测定[J].药物分析杂志,2011,31(2):217-221.Huang Z F,Yi J H,Chen D A,et al.HPLC specific chromatogram of Radix Aconiti praeparata and determination of six esteric alkaloids[J].Chinese Journal of Pharmaceutical Analysis,2011,31(2):217-221.
[6] 包同力嘎,阿如娜,马泽新,等.HPLC法测定草乌芽中新乌头碱、乌头碱、次乌头碱的含量[J].辽宁中医杂志,2016,43(9):1951-1952.Bao T L G,A R N,Ma Z X,et al.Determination of neoaconitine,aconitine and hypaconitine in Radix Aconiti kusnezoffii Buds by HPLC[J].Liaoning Journal of Traditional Chinese Medicine,2016,43(9):1951-1952.
[7] 马鑫,王巍,王耀文.HPLC法测定草乌中新乌头碱、乌头碱和次乌头碱的含量[J].实用中医内科杂志,2012,26(3):15-16.Ma X,Wang W,Wang Y W.Determination of mesaconitine,aconitine and hypaconitine in radix Aconitum kusnezoffii by HPLC[J].Journal of Practical Traditional Chinese Internal Medicine,2012,26(3):15-16.
[8] 乌力吉特古斯.草乌的民族医药学及草乌叶药理实验和化学成分研究[D].呼和浩特:内蒙古大学,2008.Uliji.Study on aconite in ethnic medico-pharmacy and on pharmacological experiment and chemical components of leaves of Aconitum kusnezoffii[D].Hohhot:Inner Mongolia University,2008.
[9] 包勒朝鲁,乌兰图雅,那生桑,等.HPLC法测定蒙医烘制草乌中的乌头碱含量[J].中华中医药学刊,2014,32(6):1363-1365.Bao L C L,Wu L T Y,Na S S,et al.Determination of aconitine content in Mongolian medicine grilled kusnezoffii by HPLC[J].Chinese Archives of Traditional Chinese Medicine,2014,32(6):1363-1365.
[10] 董忠民,李正理.北乌头种子和幼苗的发育研究[J].植物综合生物学杂志,1992,34(11):868-873.Dong Z M,Li Z L.Developmental studies on the seed and seedling of Aconitum kusnezoffii Reichb[J].Acta Botanica Sinica,1992,34(11):868-873.
[11] 朱龙章,朱丽丹,丁明开.中药材草乌高产栽培技术[J].现代农业科技,2017(13):90,94.Zhu L Z,Zhu L D,Ding M K.High-yield cultivation techniques of radix Aconiti kusnezoffii J[J].Modern Agricultural Science and Technology,2017(13):90,94.
[12] 艾洪莲,何华杰,杨曼思,等.黄草乌种子萌发特性研究[J].种子,2015,34(12):80-82.Ai H L,He H J,Yang M S,et al.Characteristics of seed germination of Aconitum vilmorinianum Komarov[J].Seed,2015,34(12):80-82.
[13] 付玉杰,赵文灏,侯春莲,等.超声提取-高效液相色谱法测定甘草中甘草酸含量[J].植物研究,2005,25(2):210-212.Fu Y J,Zhao W H,Hou C L,et al.Determination of the Glycyrrhizic acid content from Glycyrrhiza uralensis by HPLC[J].Bulletin of Botanical Research,2005,25(2):210-212.
[14] 张学科,马春慧,杨磊,等.高效液相色谱法同时测定楠竹叶提取物中的4种黄酮[J].植物研究,2013,33(4):490-493.Zhang X K,Ma C H,Yang L,et al.Simultaneous determination of 4 flavonoids in extracts of Phyllostachys pubescens leaves by HPLC[J].Bulletin of Botanical Research,2013,33(4):490-493.
[15] 昝珂,旺杰次仁,鲁静,等.HPLC测定蒙药材草乌叶中4种双酯型二萜生物碱含量[J].中国中药杂志,2018,43(4):766-771.Zan K,Wangjie C R,Lu J,et al.Content determination of four diester diterpenoid alkaloids in leaves of Aconitum kusnezoffii by HPLC[J].China Journal of Chinese Materia Medica,2018,43(4):766-771.
[16] Qiao Y L,Zhang Y H,Zhang W,et al.A rapid resolution liquid chromatographic method for fingerprint analysis of raw and processed Caowu(Aconitum kusnezoffii)[J].Journal of AOAC International,2009,92(2):653-662.
[17] 邵财,逄世峰,刘志强,等.UPLC法同时测定草乌中6种单酯和双酯型生物碱[J].中华中医药学刊,2016,34(3):693-695.Shao C,Pang S F,Liu Z Q,et al.Determination of six kinds of monoester and diester-alkaloids in radix Aconiti kusnezoffii by UPLC[J].Chinese Archives of Traditional Chinese Medicine,2016,34(3):693-695.
[18] 张艳,黄丹,刘秀秀,等.HPLC同时测定草乌中的6种生物碱[J].华西药学杂志,2017,32(6):652-654.Zhang Y,Huang D,Liu X X,et al.Simultaneous determination of six kinds of alkaloids in Aconiti kusnezoffii Radix by HPLC[J].West China Journal of Pharmaceutical Sciences,2017,32(6):652-654.
[19] 随志刚,陈明玉,刘志强,等.草乌中乌头类生物碱提取方法比较研究[J].时珍国医国药,2009,20(3):513-514.Sui Z G,Chen M Y,Liu Z Q,et al.Comparison of different extraction methods of aconitum alkaloids from radix Aconiti kusnezoffii[J].Lishizhen Medicine and Materia Medica Research,2009,20(3):513-514.
[20] 锡林通嘎拉嘎,那生桑.内蒙古中东部地区草乌乌头碱类含量分析[J].中国现代中药,2015,17(5):458-461.Xilin T G L G,Na S S.Analysis of aconitine content in Aconiti kusnezoffii radix of eastern Inner Mongolia[J].Modern Chinese Medicine,2015,17(5):458-461.

不同地区草乌种子中生物碱含量的测定与分析

Determination and Analysis of Alkaloid Content in Aconitum kusnezoffii Seeds from Different Areas

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价

[1]	邓绍立;姜国斌*;任贤;金华;马金龙;邹吉祥. 干旱胁迫下杨树嫩茎质外体内源激素的响应[J]. 植物研究, 2013, 33(6): 690-696.
[2]	孙晓莉;刘帅华;汪雷;李汶罡;赵春建*;祖元刚. 高效液相色谱法同时测定五指山绿茶中5种儿茶素类成分[J]. 植物研究, 2011, 31(3): 377-380.
[3]	张宇;姜守刚;祖元刚*;汪琢;付玉杰. 7-木糖-10-去乙酰基紫杉醇的优化试验研究[J]. 植物研究, 2010, 30(3): 369-372.
[4]	石晋;栾雨时;*;王静云;田俊平. HPLC法测定甘薯叶片中的叶黄素[J]. 植物研究, 2008, 28(6): 767-769.
[5]	张　琳;祖元刚;牛卉颖;张颜滨;孙志强. 长春花(Catharanthus roseus*)中吲哚类生物碱含量的比较[J]. 植物研究, 2008, 28(2): 240-243.
[6]	马　伟;王振月*;陈立超;王宗权;赵海鹏;王　丹. 不同光质对毛脉酸模中蒽醌类成分的影响[J]. 植物研究, 2007, 27(6): 763-769.
[7]	郑春英;平文祥;刘松梅;祖元刚. HPLC-ELSD法在刺五加药材糖类成分分析中的应用[J]. 植物研究, 2007, 27(3): 376-379.
[8]	张艳华;孙立夫;施维林;王奇超;. HPLC法测定黑龙江省不同地区刺五加紫丁香苷的含量[J]. 植物研究, 2006, 26(1): 123-126.
[9]	付玉杰, 赵文灏, 侯春莲, 刘晓娜, 施晓光, 祖元刚. 超声提取-高效液相色谱法测定甘草中甘草酸含量[J]. 植物研究, 2005, 25(2): 210-212.
[10]	方芳, 李似姣, 管雁, 诸葛磊, 郭水良. 基于3种香茶菜属药用植物高效液相色谱的主成分分析[J]. 植物研究, 2004, 24(3): 351-356.