转基因PnDof30拟南芥非生物胁迫下的抗性分析

doi:10.7525/j.issn.1673-5102.2020.03.012

植物研究 ›› 2020, Vol. 40 ›› Issue (3): 407-415.doi: 10.7525/j.issn.1673-5102.2020.03.012

转基因PnDof30拟南芥非生物胁迫下的抗性分析

张雨晴¹, 刘野¹, 曲春浦², 刘关君², 杨天天³, 杨成君¹

1. 东北林业大学林学院, 哈尔滨 150040;
2. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040;
3. 东北林业大学野生动物与自然保护地学院, 哈尔滨 150040

收稿日期:2019-10-17 出版日期:2020-05-05 发布日期:2020-05-29
通讯作者: 杨成君,E-mail:nxyycj@163.com E-mail:nxyycj@163.com
作者简介:张雨晴(1994-),女,硕士研究生,主要从事森林植物资源方面的研究。
基金资助:
中央高校基本科研业务费专项资金（2572017CA07）；黑龙江省博士后科研启动金（LBH-Q15005）

Resistance Analysis of Transgenic PnDof30 Arabidopsis under Abiotic Stress

ZHANG Yu-Qing¹, LIU Ye¹, QU Chun-Pu², LIU Guan-Jun², YANG Tian-Tian³, YANG Cheng-Jun¹

1. College of Forestry, Northest Forestry University, Harbin 150040;
2. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
3. College of Wildlife and Nature Conservation, Northeast Forestry University, Harbin 150040

Received:2019-10-17 Online:2020-05-05 Published:2020-05-29
Supported by:
This work was supported by the Fundamental Research Funds for the Central Universities(2572017CA07);Postdoctoral Scientific Research Developmental Fund of Heilongjiang Province(LBH-Q15005)

摘要/Abstract

摘要： Dof（DNA-binding with one finger）转录因子是植物中特有的一类转录因子，是锌指蛋白家族中的一个具有众多成员的家族，氨基酸长度一般在200~400，含有非常保守的N端和较为多变的C端。已有研究表明，Dof转录因子家族在参与植物发育的多种生理途径和调节碳氮代谢、增加氮素的吸收与利用，提高植株抗逆能力中起着重要作用。为了探究小黑杨（Populus simonii×P.nigra）中Dof30基因的抗逆能力，本研究以转基因PnDof30拟南芥为研究对象，对干旱、盐和渗透胁迫后过表达PnDof30拟南芥株系L2和野生型拟南芥WT的生理指标进行比较。发现胁迫后拟南芥株系L2的种子萌发率、根长和鲜重等指标均高于WT；同时SOD、POD、脯氨酸含量高于WT，叶绿素和MDA含量下降；胁迫后L2中的PnDof30基因表达量显著提高。这些结果表明了PnDof30基因具有抗旱、耐盐和渗透胁迫的能力，对全面了解Dof转录因子的抗逆胁迫功能具有重要的意义。

关键词: 小黑杨, Dof转录因子, 转基因PnDof30拟南芥, 非生物胁迫

Abstract: Dof(DNA-binding with one finger) transcription factor is a unique transcription factor in plants,it is a family of zinc finger proteins with many members,the length of amino acids is generally between 200-400, and it contains very conservative N-terminal and relatively changeable C-terminal. Previous studies have shown that Dof transcription factor family plays an important role in regulating carbon and nitrogen metabolism, improving nitrogen uptake and utilization, and enhancing abiotic stress tolerance. To explore the resistance of PnDof30 gene in Populus simonii×P.nigra, this study used transgenic PnDof30 Arabidopsis thaliana as a research object to compare the physiological and biochemical indices of over-expressed PnDof30 Arabidopsis line L2 and wild type A.thaliana under drought, salt and osmotic stress. It was found that the seed germination rate, root length and fresh weight of L2 were higher than those of WT; the contents of SOD, POD and proline were higher than those of WT, while the contents of chlorophyll and MDA were decreased; the PnDof30 gene in L2 was significantly increased after stress. These results indicate that PnDof30 gene has the ability to resist drought, salt and osmotic stress. The results of this experiment will be of great significance for understanding the stress resistance of Dof transcription factors.

Key words: Populus simonii×P.nigra, Dof transcription factor, transgenic PnDof30 Arabidopsis thaliana, abiotic stress

中图分类号:

Q949.748.3

张雨晴, 刘野, 曲春浦, 刘关君, 杨天天, 杨成君. 转基因PnDof30拟南芥非生物胁迫下的抗性分析[J]. 植物研究, 2020, 40(3): 407-415.

ZHANG Yu-Qing, LIU Ye, QU Chun-Pu, LIU Guan-Jun, YANG Tian-Tian, YANG Cheng-Jun. Resistance Analysis of Transgenic PnDof30 Arabidopsis under Abiotic Stress[J]. Bulletin of Botanical Research, 2020, 40(3): 407-415.

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转基因PnDof30拟南芥非生物胁迫下的抗性分析

Resistance Analysis of Transgenic PnDof30 Arabidopsis under Abiotic Stress

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