AgNO3逆转的黄芩组培玻璃化苗的活性成分含量及其生物活性研究

doi:10.7525/j.issn.1673-5102.2020.03.011

植物研究 ›› 2020, Vol. 40 ›› Issue (3): 401-406.doi: 10.7525/j.issn.1673-5102.2020.03.011

AgNO₃逆转的黄芩组培玻璃化苗的活性成分含量及其生物活性研究

王胜芳, 韩淑兰, 党士坤, 王慧梅

东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2019-04-08 出版日期:2020-05-05 发布日期:2020-05-29
通讯作者: 王慧梅,E-mail:whm0709@163.com E-mail:whm0709@163.com
作者简介:王胜芳(1991-),女,硕士研究生,主要从事植物组织培养及生物活性研究。
基金资助:
中央高校基本业务基金（2572017EA03）；黑龙江省自然科学基金（C2015051）

Contents of Active Components of Scutellaria baicalensis Plantlet and Its Biological Activities of Hyperhydricityin Tissue Culture Recvered by AgNO₃

WANG Sheng-Fang, HAN Shu-Lan, DANG Shi-Kun, WANG Hui-Mei

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University 150040

Received:2019-04-08 Online:2020-05-05 Published:2020-05-29
Supported by:
Special Funds for Basic Research of Central University(2572017EA03);Natural Science Fund of Heilongjiang Province(C2015051)

摘要/Abstract

摘要： 研究AgNO₃对黄芩组培玻璃化苗的逆转作用，并在此基础上对逆转的黄芩组培玻璃化苗的活性成分含量及活性进行分析。结果表明，AgNO₃对黄芩的玻璃化苗具有明显的逆转作用，且这种逆转作用受AgNO₃浓度的影响。在AgNO₃的浓度为4 mg·L^-1时，玻璃化苗的逆转率最高为88%，且逆转的黄芩苗的生长状态最好。在此基础上，对逆转1个月的黄芩玻璃化苗的活性成分含量及生物活性进行研究。逆转的黄芩玻璃化苗体内黄芩苷的含量为68.6 mg·g^-1，明显高于玻璃化苗（38.3 mg·g^-1），但低于正常的组培苗（108.2 mg·g^-1）。此外，提取物的抗氧化、抗菌和抗肿瘤实验结果显示，逆转的黄芩玻璃化组培苗明显好于玻璃化苗，但仍低于正常的组培苗。以上研究结果表明，AgNO₃对黄芩组培玻璃化苗具有较好的逆转及恢复作用。

关键词: 玻璃化, AgNO₃, 黄芩苷, 生物活性

Abstract: We studied the reversal effect of AgNO₃ on the hyperhydric Scutellaria baicalensis plantlets, and the contents of the active components. AgNO₃ had a significant recovery effect on biological activities of the restored hyperhydric S.baicalensis plantlets and the recovery effect was affected by the concentration of AgNO₃. When the concentration of AgNO₃ was 4 mg·L^-1, the recovery rate of hyperhydric plantlets was 88%, and the recovered S.baicalensis plantlets had the best growth state. We studied the contents of active components and biological activities of hyperhydric S.baicalensis plantlets recovered by AgNO₃ for one month. The content of baicalin in recovered hyperhydric S.baicalensis plantlets was 68.6 mg·g^-1, significantly higher than that in hyperhydric plantlets(38.3 mg·g^-1), but lower than that in normal tissue cultured plantlets(108.2 mg·g^-1). The results of antioxidant, antimicrobial and anti-tumor experiments showed that the recovered hyperhydric S.baicalensis plantlets were significantly better than the hyperhydric plantlets, but still lower than the normal tissue-cultured plantlets. Therefore, AgNO₃ had a good recovery effect on the hyperhydric plantlets of S.baicalensis.

Key words: hyperhydricity, AgNO₃, baicalin, biological activities

中图分类号:

Q949.777.6

王胜芳, 韩淑兰, 党士坤, 王慧梅. AgNO₃逆转的黄芩组培玻璃化苗的活性成分含量及其生物活性研究[J]. 植物研究, 2020, 40(3): 401-406.

WANG Sheng-Fang, HAN Shu-Lan, DANG Shi-Kun, WANG Hui-Mei. Contents of Active Components of Scutellaria baicalensis Plantlet and Its Biological Activities of Hyperhydricityin Tissue Culture Recvered by AgNO₃[J]. Bulletin of Botanical Research, 2020, 40(3): 401-406.

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AgNO₃逆转的黄芩组培玻璃化苗的活性成分含量及其生物活性研究

Contents of Active Components of Scutellaria baicalensis Plantlet and Its Biological Activities of Hyperhydricityin Tissue Culture Recvered by AgNO₃

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