大青杨PubZIP1基因的克隆及亚细胞定位与抗旱表达特性分析

doi:10.7525/j.issn.1673-5102.2020.02.010

摘要/Abstract

摘要： 从大青杨中克隆得到PubZIP1基因，通过基因测序结果可知，PubZIP1基因全长1 083 bp，编码360个氨基酸。分析得出PubZIP1蛋白含有BZIP和DOG1两个结构域，其二级结构包括α-螺旋（62.50%）、无规卷曲（29.72%）、延伸链（5.56%）、β-折叠（2.22%）。通过亚细胞定位试验表明PubZIP1基因位于细胞核。通过qRT-PCR分析表明，在模拟干旱的不同7% PEG6000胁迫时间下，分析结果表明胁迫后PubZIP1基因在大青杨根中的表达量呈下降趋势。而在大青杨茎和叶片中的表达量呈上升趋势，尤其是在叶片中明显被诱导表达。预测该基因可能主要在叶片中表达并行使功能。

关键词: 大青杨, PubZIP1基因, 生物信息学, 亚细胞定位

Abstract: The PubZIP1 gene was cloned from Populus ussuriensis Kom. The results of gene sequencing showed that the PubZIP1 gene has 1 083 bp coding sequences which encodes a polypeptide of 360 amino acids. By the conserved domains analysis, there are conserved BZIP and DOG1 in PubZIP1 protein. And its secondary structure consists of the 62.50% α-helix, 29.72% random coil, 5.56% extended strand and β-sheet(2.22%). Subcellular localization analysis of the PubZIP1 gene indicates it localized in the nucleus. The result of qRT-PCR analysis showed that the expression of PubZIP1 was decreased after PEG6000 treatment, while it was increased in stem and leaves, especially for leaves. It was predicted that this gene maybe mainly express and function in leaves.

Key words: Populus ussuriensis Kom., PubZIP1 gene, bioinformation, subcellular localization

中图分类号:

S722.3+6

刘晓, 杨佳, 张馨, 马苗苗, 杨静莉. 大青杨PubZIP1基因的克隆及亚细胞定位与抗旱表达特性分析[J]. 植物研究, 2020, 40(2): 233-242.

LIU Xiao, YANG Jia, ZHANG Xin, MA Miao-Miao, YANG Jing-Li. PubZIP1 Gene Cloning,Subcellular Localization and Expression Pattern under Drought Stress[J]. Bulletin of Botanical Research, 2020, 40(2): 233-242.

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