滇楸种质生长性状遗传变异及表型性状遗传多样性分析

doi:10.7525/j.issn.1673-5102.2018.06.007

摘要/Abstract

摘要： 为明确滇楸种质生长和表型性状的遗传变异程度及遗传多样性大小，提出合理的杂交育种策略。本研究对滇楸20个无性系进行了连年的生长性状测定，测量了其叶长、叶宽、皮孔长、皮孔宽、皮孔密度等表型性状。研究结果表明滇楸种质1、3、4和5 a树高及1~6 a胸径在无性系间差异均达显著水平。树高遗传变异系数和重复力随年份变化波动较大，胸径遗传变异系数和重复力年份间较为稳定，且重复力较高。表明滇楸种质胸径受遗传控制程度较大，且稳定性更高。滇楸种质叶长和叶宽遗传多样性指数较高，分别为2.016和2.012。皮孔性状遗传变异系数较高，皮孔长、皮孔面积和皮孔密度表型变异系数均超过20%，遗传变异系数均超过15%。说明滇楸种质表型变异较为丰富，具有较好的遗传改良基础。相关分析结果表明皮孔密度与生长呈负相关，皮孔大小与生长呈正相关。聚类分析将滇楸种质划分为4类，第Ⅰ类皮孔面积最大，密度最小；第Ⅱ类生长最慢，叶长最大；第Ⅲ类生长最快，叶形最宽；第Ⅳ类叶柄最长。

关键词: 滇楸, 表型性状, 遗传变异, 遗传多样性

Abstract: The experiment was conducted to study genetic variation and genetic diversity of Catalpa fargesii f. duclouxii germplasm and put forward reasonable cross breeding strategy. The perennial growth traits and the leaf length, leaf width, lenticel length, lenticel width, lenticel density of 20 clones were measured. The results showed that 1, 2, 3, 4 and 5 a height and 1-6 a DBH were significantly different among clones. Repeatability and genetic variation coefficient of height changed in volatility with years change, however, those parameters of DBH were relatively stable. Repeatability of DBH was higher than height. It indicated that the degree of genetic control of DBH was stronger than height. The genetic diversity index of leaf length and leaf width was high, they were 2.016 and 2.012, respectively. Phenotypic variation coefficients of lenticel length, area and density were more than 20% and the genetic variation coefficient were more than 15%. There was ample morphological diversity among C.fargesii f. duclouxii clones. By the correlation analysis, the lenticel density was negatively correlated with growth, but lenticel size and growth traits had positive correlation. The germplasms were divided into four types by cluster analysis, the first type had maximum lenticel area and the minimum lenticel density; type Ⅱ grew the most slowly, but it had maximun leaf length; type Ⅲ had fastest growth rate and maximun leaf width; and the type Ⅳ petioles were longest.

Key words: Catalpa fargesii f. duclouxii, phenotypic traits, genetic variation, genetic diversity

中图分类号:

S792.21

肖遥, 麻文俊, 易飞, 杨桂娟, 王秋霞, 王平, 王军辉. 滇楸种质生长性状遗传变异及表型性状遗传多样性分析[J]. 植物研究, 2018, 38(6): 843-851.

XIAO Yao, MA Wen-Jun, YI Fei, YANG Gui-Juan, WANG Qiu-Xia, WANG Ping, WANG Jun-Hui. Genetic Variation of Growth Traits and Genetic Diversity of Phenotypic Traits in Catalpa fargesii f. duclouxii Germplasm[J]. Bulletin of Botanical Research, 2018, 38(6): 843-851.

参考文献

1. 潘庆凯,康平生,郭明. 楸树[M]. 北京:中国林业出版社,1991:37-38.Pan Q K,Kang P S,Guo M. Catalpa bungei[M]. Beijing:Chinese Forestry Press,1991:37-38.
2. 姚淑均,张守攻,王军辉,等. 滇楸花部性状的表型多样性研究[J]. 中南林业科技大学学报,2013,33(10):19-24.Yao S J,Zhang S G,Wang J H,et al. Study on phenotypic diversity of flower characters in Catalpa fargesii f. duclouxii[J]. Journal of Central South University of Forestry & Technology,2013,33(10):19-24.
3. 黄国伟,陈慧玲,包一鸣,等. 滇楸幼苗无性系光合特性的比较[J]. 东北林业大学学报,2015,43(1):20-23,66.Huang G W,Chen H L,Bao Y M,et al. Photosynthetic characteristics of five Catalpa duclouxii seedling clones[J]. Journal of Northeast Forestry University,2015,43(1):20-23,66.
4. 张新叶,李振芳,张亚东,等. 湖北引种滇楸优良无性系早期选择[J]. 中国农学通报,2016,32(10):24-29.Zhang X Y,Li Z F,Zhang Y D,et al. Early stage selection of fine clones of Catalpa fargesii f. duclouxii in Hubei Province[J]. Chinese Agricultural Science Bulletin,2016,32(10):24-29.
5. 马庆华,王贵禧,梁维坚,等. 我国榛属植物种质资源的研究、利用与创新[J]. 果树学报,2013,30(1):159-164.Ma Q H,Wang G X,Liang W J,et al. Research progresses,germplasm utilization and breeding innovation of Corylus in China[J]. Journal of Fruit Science,2013,30(1):159-164.
6. 赵爽,赵福洞,石鹤飞,等. 核桃品种绿岭杂交后代主要性状的遗传分析与优株选择研究[J]. 植物遗传资源学报,2015,16(5):1004-1012.Zhao S,Zhao F D,Shi H F,et al. Main traits genetic analysis and superior tree selection of hybrid Offsprings of ‘Lvling’ Walnut[J]. Journal of Plant Genetic Resources,2015,16(5):1004-1012.
7. 梅芳,王军辉,贾继文,等. 楸树种质生长和叶部性状的遗传多样性[J]. 植物遗传资源学报,2014,15(1):206-210,214.Mei F,Wang J H,Jia J W,et al. Genetic diversity of growth and leaf traits of Catalpa bungei[J]. Journal of Plant Genetic Resources,2014,15(1):206-210,214.
8. Chung J D,Nigh G,Chien C T,et al. Genetic variation and tree improvement of konishii fir(Cunninghamia lanceolata (Lamb.) Hook. var. konishii)in Taiwan[J]. Silvae Genetica,2011,60(5):196-205.
9. 樊汝汶,吴琼美,邹惠渝. 滇楸 Catalpa fargesii f. duclouxii (Dode)Gilmour的胚胎学研究[J]. 南京林业大学学报:自然科学版,1980,4(2):67-80.Fan R W,Wu Q M,Zou H Y. Embryological Studies of Catalpa fargesii f. duclouxii (Dode) Gilmour[J]. Journal of Nanjing Forestry University:Natural Sciences,1980,4(2):67-80.
10. 杨安敏,姚淑均,许杰,等. 滇楸半同胞家系子代苗期性状的遗传变异[J]. 种子,2010,29(3):89-90,93.Yang A M,Yao S J,Xu J,et al. The genetic variation of seeding traits among half-sib familes of pius trees of Catalpa duclouxii dode[J]. Seed,2010,29(3):89-90,93.
11. 吴倩婷,顾沈华,王婉,等. 沿海地区滇楸无性系幼龄林对比测定及选择研究[J]. 中国农学通报,2014,30(16):53-56.Wu Q T,Gu S H,Wang W,et al. Comparative observation and clones choosing of two-year Catalpa duclouxii dode clones forest in coastal area[J]. Chinese Agricultural Science Bulletin,2014,30(16):53-56.
12. 王军辉,张守攻,麻文俊,等. 一种楸树无性系的选育方法:中国,CN102939898A[P]. 2013-02-27.
13. 贠慧玲,王军辉,张宋智,等. 楸树无性系表型识别特征与遗传变异[J]. 东北林业大学学报,2012,40(2):34-38,46.Yun H L,Wang J H,Zhang S Z,et al. Genetic variation and identification of phenotypic characteristics of clones of Catalpa bungei[J]. Journal of Northeast Forestry University,2012,40(2):34-38,46.
14. 林元震. R与ASReml-R统计学[M]. 北京:中国林业出版社,2017.Liu Y Z. R and ASReml-R statistics[M]. Beijing:Chinese Forestry Press,2017.
15. Keylock C J. Simpson diversity and the shannon-wiener index as special cases of a generalized entropy[J]. Oikos,2010,109(1):203-207.
16. 陈晓阳,沈熙环. 林木育种学[M]. 北京:高等教育出版社,2005.Chen X Y,Shen X H. Forest tree breeding[M]. Beijing:Higher Education Press,2005.
17. 续九如. 林木数量遗传学[M]. 北京:高等教育出版社,2006.Xu J R. Quantitative genetics in forestry[M]. Beijing:Higher Education Press,2006.
18. Hodge G R,Dvorak W S. The CAMCORE international provenance/progeny trials of Gmelina arborea:genetic parameters and potential gain[J]. New Forests,2004,28(2-3):147-166.
19. Goh D K S,Japarudin Y,Alwi A,et al. Growth differences and genetic parameter estimates of 15 teak(Tectona grandis L. f.)genotypes of various ages clonally propagated by microcuttings and planted under humid tropical conditions[J]. Silvae Genetica,2013,62(1-6):196-206.
20. 张一,储德裕,金国庆,等. 马尾松1代育种群体遗传多样性的ISSR分析[J]. 林业科学研究,2009,22(6):772-778.Zhang Y,Chu D Y,Jin G Q,et al. Molecular characterization of elite genotypes within a first generation breeding population of Pinus massoniana using ISSR[J]. Forest Research,2009,22(6):772-778.
21. 辛娜娜,张蕊,徐肇友,等. 木荷1代育种群体遗传多样性分析[J]. 林业科学研究,2015,28(3):332-338.Xin N N,Zhang R,Xu Z Y,et al. Genetic diversity among breeding parents of Schima superba revealed by SSR[J]. Forest Research,2015,28(3):332-338.
22. 王辉,李双铃,任艳,等. 利用AhMITE转座子分子标记研究花生栽培种及高世代材料的亲缘关系[J]. 农业生物技术学报,2013,21(10):1176-1184.Wang H,Li S L,Ren Y,et al. Genetic relationship of peanut(Arachis hypogaea L.)varieties and advanced generation lines evaluated by AhMITE transposable markers[J]. Journal of Agricultural Biotechnology,2013,21(10):1176-1184.
23. Mikola J,Paaso U,Silfver T,et al. Growth and genotype×environment interactions in Betula pendula:can tree genetic variation be maintained by small-scale forest ground heterogeneity?[J]. Evolutionary Ecology,2014,28(5):811-828.
24. 孙晓梅,张守攻,侯义梅,等. 短轮伐期日本落叶松家系生长性状遗传参数的变化[J]. 林业科学,2004,40(6):68-74.Sun X M,Zhang S G,Hou Y M,et al. Age trends of genetic parameters for growth traits in short rotation Larix kaempferi families[J]. Scientia Silvae Sinicae,2004,40(6):68-74.
25. 郑仁华,苏顺德,肖晖,等. 杉木优树多父本杂交子代测定及母本选择[J]. 林业科学,2014,50(9):44-50.Zheng R H,Su S D,Xiao H,et al. Female selection based on a polycross progeny test of Cunninghamia lanceolata plus trees[J]. Scientia Silvae Sinicae,2014,50(9):44-50.
26. 王建忠,熊涛,张磊,等. 25年生大花序桉种源生长与形质性状的遗传变异及选择[J]. 林业科学研究,2016,29(5):705-713.Wang J Z,Xiong T,Zhang L,et al. Genetic variation and selection on growth and stem form quality traits of 25-year-old Eucalyptus cloeziana provenance[J]Forest Research,2016,29(5):705-713.
27. 赵爽,任俊杰,石鹤飞,等. 早实核桃叶片性状遗传规律研究[J]. 植物遗传资源学报,2015,16(3):659-663.Zhao S,Ren J J,Shi H F,et al. Study on leaf traits genetic law of precocious walnut[J]. Journal of Plant Genetic Resources,2015,16(3):659-663.
28. 刘宇,徐焕文,边秀艳,等. 白桦半同胞家系苗期生长和光合特性及其选育评价指标筛选[J]. 西北植物学报,2013,33(5):963-969.Liu Y,Xu H W,Bian X Y,et al. Selection of seedling growth and photosynthetic traits assistant evaluated factors in Betula platyphylla half-sib families[J]. Acta Botanica Boreali-Occidentalia Sinica,2013,33(5):963-969.
29. 刘殿昆,刘梦然,李志新,等. 转 TaLEA 基因小黑杨无性系生长性状变异研究[J]. 植物研究,2015,35(4):540-546.Liu D K,Liu M R,Li Z X,et al. Variation analysis of growth traits of transgenic Populus simonii×P. nigra clones carrying TaLEA gene[J]. Bulletin of Botanical Research,2015,35(4):540-546.

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