转BpCUCt白桦生长特征及内源IAA含量的变化

doi:10.7525/j.issn.1673-5102.2018.04.004

摘要/Abstract

摘要： 为了揭示白桦BpCUCt基因的功能，分别构建了BpCUC2启动子及35S启动子驱动的BpCUCt载体，以白桦合子胚为受体开展转基因工作，观察转基因株系表型变化，并测定转基因株系和WT的内源激素IAA含量。结果表明，对比转基因株系生长特性可知，转BpCUCt基因白桦株系比WT矮小，生长也比较缓慢，转基因株系节间距均变长，而ProCUC2：：BpCUCt转基因株系叶片边缘比WT叶片平滑，35S：：BpCUCt转基因株系叶边缘与野生型并无明显区别。由转基因白桦和WT的内源激素IAA含量可知，83.33%的转基因株系左侧叶片、右侧叶片及主脉IAA含量均显著高于WT株系。表明BpCUCt基因在一定程度上与BpCUC2基因表现出相同的功能，同时改变白桦内源生长素IAA的含量。

关键词: 白桦, BpCUCt, BpCUC2, 内源激素IAA

Abstract: To identify the function of BpCUCt in birch, we constructed two vectors that could be expressed in plants, namely ProCUC2::BpCUCt and 35S::BpCUCt. Meanwhile, we regarded zygotic embryo from elite birch trees as transgenic receptor, observed the changed phenotypes of transgenic lines and measured the endogenous IAA content. Compared to the wild type, the BpCUCt transgenic lines were with smaller height, lower growth characters and longer knot distances. However, the leaf margin of ProCUC2::BpCUCt lines were smoother than the wild type, transgenic lines of 35S::BpCUCt did not distinguish from the wild type. In view of the endogenous IAA content from the transgenic lines and wild type, the 83.33% of transgenic lines that was with more endogenous IAA content than wild type in the left leaf, the right leaf and the main vein. In conclusion, to some degree, the function of BpCUCt played similar roles in the function of BpCUC2, meanwhile, this gene was also related to endogenous IAA content in birch trees.

Key words: Betula platyphylla, BpCUCt, BpCUC2, endogenous IAA

中图分类号:

S792.153

陈晨, 邢宝月, 边秀艳, 许思佳, 刘桂丰, 姜静. 转BpCUCt白桦生长特征及内源IAA含量的变化[J]. 植物研究, 2018, 38(4): 506-517.

CHEN Chen, XING Bao-Yue, BIAN Xiu-Yan, XU Si-Jia, LIU Gui-Feng, JIANG Jing. Growth Characteristics and Endogenous IAA Content of BpCUCt Transgenic Lines in Betula platyphylla[J]. Bulletin of Botanical Research, 2018, 38(4): 506-517.

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