高速匀质-微波辅助提取红松籽油工艺及其品质评价

doi:10.7525/j.issn.1673-5102.2017.05.020

植物研究 ›› 2017, Vol. 37 ›› Issue (5): 789-796.doi: 10.7525/j.issn.1673-5102.2017.05.020

高速匀质-微波辅助提取红松籽油工艺及其品质评价

杨明非¹, 苏雯², 寇萍³, 王希清³, 郭娜³, 臧玉萍³, 付玉杰³

1. 北日本制药株式会社产品研发室, 日本富山县中新川郡上市町若杉55号 930-0314;
2. 广东省珠海市斗门区公立医疗卫生机构管理中心, 珠海 519125;
3. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2017-03-07 出版日期:2017-09-15 发布日期:2017-09-15
通讯作者: 付玉杰,E-mail:yujie_fu2002@yahoo.com E-mail:yujie_fu2002@yahoo.com
作者简介:杨明非(1989-),男,临床医学硕士,主要从事肿瘤药物研究。
基金资助:
十三五国家重点研发计划项目（2016YFD0600805）

Ultra Turrax-Microwave Assisted Extraction of Oil from Pinus koraiensis Sieb Seed and its Quality Evaluation

YANG Ming-Fei¹, SU Wen², KOU Ping³, WANG Xi-Qing³, GUO Na³, ZANG Yu-Ping³, FU Yu-Jie³

1. Research and Development Department of Kitanihon Pharmaceutical CO., No. 55, Kamiichi, Nakaniikawa District, Toyama, Japan 930-0314;
2. Management Center of Public Medical and Health Institutions in Doumen District, Zhuhai City, Guangdong Province, Zhuhai 519125;
3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040

Received:2017-03-07 Online:2017-09-15 Published:2017-09-15
Supported by:
13th Five-Year The National Key Research Projects(2016YFD0600805)

摘要/Abstract

摘要： 以红松籽仁为原料，以无水乙醇为提取溶剂，优化高速匀质-微波辅助提取红松籽油的工艺，考察了匀质速度、匀质时间、液料比、微波温度、微波功率及微波时间对红松籽油提取率的影响，最终确定了红松籽油最佳提取条件为：匀质速度12 000 r·min^-1，匀质时间120 s，液料比20 mL·g^-1，微波温度60℃，微波功率700 W，微波时间50 min。在上述条件下，红松籽油最优提取率可达60.3%。利用GC-MS对得到的红松籽油的脂肪酸成分进行了分析，其主要成分为棕榈酸、皮诺敛酸、亚油酸、反-13-十八碳烯酸、顺-13-十八碳烯酸、硬脂酸，不饱和脂肪酸含量高达85.55%，其中松籽油所含有的特异性不饱和脂肪酸-皮诺敛酸含量可达13.65%。此外，对该工艺提取得到的红松籽油的抗氧化活性及理化性质进行了评估，发现红松籽油清除DPPH自由基能力强，其IC₅₀值为0.095 4 g·mL^-1。此外，红松籽油过氧化值和酸值较低，碘值较高，表明红松籽油是一种品质优良的天然油脂。

关键词: 红松籽油, 高速匀质-微波辅助提取, 不饱和脂肪酸, 品质评价

Abstract: In this work, ultra turrax-microwave assisted extraction process was conducted to extract oil from Pinus koraiensis using ethanol as the extraction solvent. The effects of homogenization speed, homogenization time, liquid-solid ratio, microwave temperature, microwave power, and microwave time on the oil yield were investigated. The optimal conditions for the oil extraction from P.koraiensis seed were obtained as follows:homogeneous speed 12 000 r·min^-1, homogeneous time 120 s, the ratio of liquid to material 20 mL·g^-1, microwave temperature 60℃, microwave power 700 W and microwave time 50 min. Under the above conditions, the optimum oil yield was 60.3%. The fatty acid composition of P.koraiensis seed oil was analyzed by GC-MS, and the main components were identified as linoleic acid, stearic acid and palmitic acid. The contents of unsaturated fatty acid and specific pinolenic acid reached 85.55% and 13.65%, respectively. The antioxidant activity and physicochemical properties of P.koraiensis seed oil were also determined. The seed oil exhibited better DPPH radical scavenging activity with the IC₅₀ value of 0.0954 g·mL^-1. And the seed oil was a high quality natural oil with lower acid value, lower peroxide value and high iodine value.

Key words: Pinus koraiensis Sieb seed oil, ultra turrax-microwave assisted extraction, unsaturated fatty acid, quality evaluation

中图分类号:

S791.247

杨明非, 苏雯, 寇萍, 王希清, 郭娜, 臧玉萍, 付玉杰. 高速匀质-微波辅助提取红松籽油工艺及其品质评价[J]. 植物研究, 2017, 37(5): 789-796.

YANG Ming-Fei, SU Wen, KOU Ping, WANG Xi-Qing, GUO Na, ZANG Yu-Ping, FU Yu-Jie. Ultra Turrax-Microwave Assisted Extraction of Oil from Pinus koraiensis Sieb Seed and its Quality Evaluation[J]. Bulletin of Botanical Research, 2017, 37(5): 789-796.

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高速匀质-微波辅助提取红松籽油工艺及其品质评价

Ultra Turrax-Microwave Assisted Extraction of Oil from Pinus koraiensis Sieb Seed and its Quality Evaluation

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