84K杨愈伤组织再生体系和直接分化再生体系遗传转化的比较性研究

doi:10.7525/j.issn.1673-5102.2017.04.009

植物研究 ›› 2017, Vol. 37 ›› Issue (4): 542-548.doi: 10.7525/j.issn.1673-5102.2017.04.009

84K杨愈伤组织再生体系和直接分化再生体系遗传转化的比较性研究

王丽娜, 王玉成, 杨传平

东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2017-02-02 出版日期:2017-07-15 发布日期:2017-07-22
通讯作者: 杨传平,E-mail:yangcp@nefu.edu.cn E-mail:yangcp@nefu.edu.cn
作者简介:王丽娜(1979-),女,讲师,主要从事林木基因组研究。
基金资助:
中央高校基本科研业务费专项资金项目（2572016BA02）；哈尔滨市应用技术研究与开发项目（青年后备人才A类）（2016RAQXJ065）资助

The Comparative study of Callus and Direct Differation Regenaration System of 84K Poplar

WANG Li-Na, WANG Yu-Cheng, YANG Chuan-Ping

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040

Received:2017-02-02 Online:2017-07-15 Published:2017-07-22
Supported by:
Fundamental Research Funds for the Central Universities(2572016BA02);Harbin applied technology research and development project(2016RAQXJ065)

摘要/Abstract

摘要： 植物转基因必须以建立良好的植物转化受体系统为基础，而良好的受体系统必须以具有高频率的转化率及较强的再生能力为前提。实验以84K杨叶片为材料研究其在不同培养条件、不同种类及浓度的植物激素对其脱分化及再分化的影响，并建立了两种成熟的组织再生系统，得出组织培养体系与遗传转化体系的受体系统在激素配比方面存在一定差距，所以在此基础上进行了PCK1、PCK2两个基因的愈伤途径和不定芽途径途径的遗传转化，并对两种遗传转化体系进行了对比性分析并总结出两种体系的优缺点。

关键词: 84K杨, 愈伤组织, 直接分化, 遗传转化

Abstract: High-frequent transformation and strong regeneration ability of plants are prerequisites to the establishment of good plant transformation receptor system, which is the foundation for plant genetic transformation. Taking 84K poplar leaves as study material, this experiment studies the influence of different types and concentrations of plant hormones to its dedifferentiation and re-differentiation under different cultivation conditions, and sets two mature plant tissue cultivation regeneration system. Based on above,here establishes genetic transformation system of adventitious buds and callus pathway, respectively as callus regeneration system and regeneration system of direct differentiation. Then through conducting comparative analysis of two tissue culture system using PCK1 and PCK2, here summarizes the advantages and disadvantages of two methods.

Key words: Poplar 84K, callus, direct differation, genetic transformation

中图分类号:

王丽娜, 王玉成, 杨传平. 84K杨愈伤组织再生体系和直接分化再生体系遗传转化的比较性研究[J]. 植物研究, 2017, 37(4): 542-548.

WANG Li-Na, WANG Yu-Cheng, YANG Chuan-Ping. The Comparative study of Callus and Direct Differation Regenaration System of 84K Poplar[J]. Bulletin of Botanical Research, 2017, 37(4): 542-548.

参考文献

1. Parsons T J,Sinkar V P,Stettler R F,et al. Transformation of Poplar by Agrobacterium tumefaciens[J]. Nature Biotechnology,1986,4(6):533-536.
2. 赵清爽. 84K杨组织培养及基因转化的研究[D]. 西安:陕西师范大学,2003.Zhao Q S. Studies on the tissue culture and gene transformation of Poplar 84[D]. Xi’an:Shanxi teacher’s university,2003.
3. 周熙莹. 84K杨再生和遗传转化体系的研究[D]. 哈尔滨:东北林业大学,2013.Zhou X Y. Research on 84K populus regeneration and genetic transformation system[D]. Haerbin:Northeast Forestry University,2013.
4. Liesebach M,Naujoks G. Approaches on vegetative propagation of difficult-to-root Salix caprea[J]. Plant Cell Tissue and Organ Culture,2004,79:239-247.
5. 王蒂. 植物组织培养[M]. 北京:中国农业出版社,2004:12-18.Wang D. The tissue culture in plant[M]. Beijing:China Agriculture Press,2004:12-18.
6. Jin J Z,Liang S,Hui C,et al. An efficient Agrobacterium -mediated transformation method for the edible mushroom Hypsizygus marmoreus[J]. Microbiological Research,2014,169(9-10):741-748.
7. Kayani W K,Fattahi M,Palazòn J,et al. Comprehensive screening of influential factors in the Agrobacterium tumefaciens-mediated transformation of the Himalayan elixir:Ajuga bracteosa,Wall. ex. Benth[J]. Journal of Applied Research on Medicinal & Aromatic Plants,2016,3(4):151-159.
8. Kalbande B B,Patil A S. Plant tissue culture independent Agrobacterium tumefaciens,mediated In-planta,transformation strategy for upland cotton(Gossypium hirsutum)[J]. Journal of Genetic Engineering & Biotechnology,2016,14(1):9-18.
9. Rodrigues M B C,Fávaro L C D L,Pallu A P D S,et al. Agrobacterium -mediated transformation of Guignardia citricarpa:An efficient tool to gene transfer and random mutagenesis[J]. Fungal Biology,2013,117(8):556-568.
10. 付成华,沈宝仙. I-72杨再生体系的建立[J]. 华中农业大学学报,2006,25(5):564-566.Fu C H,Shen B X. Establishment of Plant Regeneration of Populus euramericana San Martino[J]. Journal of Huazhong Agricultural University,2006,25(5):564-566.
11. Ziemienowicz A. Agrobacterium -mediated plant transformation:Factors,applications and recent advances[J]. Biocatalysis & Agricultural Biotechnology,2014,3(4):95-102.
12. Martins P K,Ribeiro A P,Kobayashi A K,et al. A simple and highly efficient Agrobacterium -mediated transformation protocol for Setaria viridis[J]. Biotechnology Reports,2015,6:41-44.
13. 赵华燕,卢善发. 杨树的组织培养及其基因工程研究植物学通[J]. 植物学通报,2001,18(2):169-176.Zhao H Y,Lu S F. Studies on Tissue Culture and Gene Engineering of Poplar[J]. Chinese Bulletin of Botany,2001,18(2):169-176.
14. Mallesham Bulle. Agrobacterium tumefaciens-Mediated transformation of Woodfordia fruticosa[J]. Journal of Genetic Engineering and Biotechnology,2015,13(2):201-207.
15. 张颖,王猛,柴娜. 杨树叶片离体再生系统的构建[J]. 山东农业科学,2010,5(37):8-10.Zhang Y,Wang M,Chai N. Establishment of invitro Regenaration system of Poplar leaves[J]. Shandong Agriculture Sciences. 2010,5(37):8-10.
16. 于志水. 白杨无性系茎尖培养及体细胞胚胎发生技术研究[D]. 北京:北京林业大学,2005.Yu Z S. Shoot culure and somatic embryogenesis in Aspen Clones[D]. Beijing:Beijing Forestry University,2005.
17. 孟庆敏. 复叶槭组织培养再生体系的建立[D]. 哈尔滨:东北林业大学,2006.Meng Q M. Establishment of Plant Regeneration system cultured in vitro of Acer negundo L. [D]. Haerbin:Northeast Forestry University,2006.

[1]	何旭, 高源, 张群野, 周晨光, 李伟, 李爽. 白城小黑杨遗传转化体系建立及其应用[J]. 植物研究, 2023, 43(5): 667-678.
[2]	杜金霞, 申婷婷, 王浩然, 林一萍, 李慧玉, 张连飞. 白桦BpSPL9基因抑制表达载体的构建及遗传转化研究[J]. 植物研究, 2023, 43(1): 30-35.
[3]	李登高, 林睿, 穆青慧, 周娜, 张焱如, 白薇. 马铃薯StNPR4基因的克隆与功能分析[J]. 植物研究, 2022, 42(5): 821-829.
[4]	任毓辉, 聂帅, 彭春雪, 杨玲, 沈海龙. 红松胚性愈伤组织增殖的激素配比、糖源类型和增殖周期效应研究[J]. 植物研究, 2022, 42(4): 704-712.
[5]	高启阳, 黄宇龙, 郭文冰, 赵奋成, 刘阳. 碳源等因素对湿地松优良无性系胚性愈伤组织增殖的影响[J]. 植物研究, 2022, 42(1): 21-28.
[6]	田红梅, 郭霄, 王奎玲, 孙迎坤. 耐冬山茶子叶再生体系的研究[J]. 植物研究, 2021, 41(3): 321-328.
[7]	袁云香. 小果卫矛愈伤组织诱导及植株再生体系的建立[J]. 植物研究, 2020, 40(5): 673-678.
[8]	杜一鸣, 钟原, 尚宏芹, 成仿云. “凤丹”牡丹花丝愈伤组织诱导及分化研究[J]. 植物研究, 2020, 40(4): 514-522.
[9]	张磊, 熊欢欢, 曹庆, 赵佳丽, 张含国. 瞬时遗传转化长白落叶松NAC基因植株抗旱性的研究[J]. 植物研究, 2020, 40(3): 394-400.
[10]	丁宝娟, 安利佳, 苏乔. 过表达钾转运蛋白基因trkH提高玉米的钾营养[J]. 植物研究, 2020, 40(1): 141-147.
[11]	樊二勤, 刘彩霞, 付鹏跃, 杨传平, 曲冠证. 84K杨PagC3H3基因表达模式分析[J]. 植物研究, 2019, 39(4): 521-528.
[12]	孙晓莎, 王遂, 赵曦阳, 曲冠证. 84K杨4CL3/4CL5基因克隆及生物信息学分析[J]. 植物研究, 2019, 39(4): 547-556.
[13]	张璐, 潘远智, 刘柿良, 陈源, 申理嘉. 宜昌百合胚性愈伤组织诱导及植株再生体系的研究[J]. 植物研究, 2019, 39(3): 338-346.
[14]	颜斌, 武丹阳, 李慧玉. 白桦BpBEE2基因的遗传转化及抗逆性分析[J]. 植物研究, 2019, 39(2): 287-293.
[15]	葛晓兰, 刘彩霞, 张鑫鑫, 李莹, 曲冠证. 柽柳ThDUF106基因的植物表达载体构建及其功能验证[J]. 植物研究, 2018, 38(2): 260-267.

84K杨愈伤组织再生体系和直接分化再生体系遗传转化的比较性研究

The Comparative study of Callus and Direct Differation Regenaration System of 84K Poplar

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价