耐冬山茶抗寒基因CjCor1的全长克隆与表达分析

doi:10.7525/j.issn.1673-5102.2016.05.017

植物研究 ›› 2016, Vol. 36 ›› Issue (5): 753-759.doi: 10.7525/j.issn.1673-5102.2016.05.017

耐冬山茶抗寒基因CjCor1的全长克隆与表达分析

杨凯^1,2, 孙迎坤², 王奎玲², 刘庆华², 高捍东¹

1. 南京林业大学, 南京 210037;
2. 青岛农业大学, 青岛 266109

收稿日期:2016-05-16 出版日期:2016-09-15 发布日期:2016-09-27
通讯作者: 高捍东,E-mail:gaohd@njfu.edu.cn E-mail:gaohd@njfu.edu.cn
作者简介:杨凯(1975-),男,讲师,博士研究生,主要从事林木种苗和观赏植物育种研究。
基金资助:
山东省高等学校科技计划项目（J13LE16）；“十二五”农村领域国家科技计划课题（2012BAD01B0703）

Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica(Nai Dong)

YANG Kai^1,2, SUN Ying-Kun², WANG Kui-Ling², LIU Qing-Hua², GAO Han-Dong¹

1. Nanjing Forestry University, Nanjing 210037;
2. Qingdao Agricultural University, Qingdao 266109

Received:2016-05-16 Online:2016-09-15 Published:2016-09-27
Supported by:
Colleges and universities science & technology plan in Shandong province(No.J13LE16);National Science & Technology Pillar Program during the Twelfth Five-year Plan Period(No.2012BAD01B0703)

摘要/Abstract

摘要： COR基因是植物冷驯化过程中重要的功能基因。为研究Cor基因在耐冬山茶（Camellia japonica）抗寒方面的功能，采取同源克隆的方法，从自然低温条件下的耐冬山茶叶片中分离到了617 bp的耐冬山茶同源COR基因全长序列，命名为CjCor1。该序列全长完整，含有600 bp的开放阅读框。氨基酸序列分析显示，CjCor1基因编码的199个氨基酸蛋白质，含有冷驯化蛋白家族WCOR413 Superfamily特有的保守域结构，在同属物种中的保守性极高。荧光定量PCR结果表明，在低温（0℃以下时）条件下，相比南方山茶原种和常见栽培品种，CjCor1基因在耐冬山茶中具有较高表达量，且在耐寒性高的品种中的表达量也较高。

关键词: 耐冬山茶, CjCor1基因, 序列分析, 基因表达

Abstract: COR genes play important roles in plant cold acclimation process. In order to study the Cor gene function of Camellia japonica to severe cold, adopting homologous cloning method, a 617 bp full-length cDNA sequence was cloned from leaves of C.japonica(Nai Dong) under the natural low temperature condition, named CjCor1. The total length sequence is complete, and contains an opening reading frame of 600 bp. Amino acids sequence alignment shows that CjCor1 gene encodes a protein of 199 amino acids, containing specific conservative domain structure of WCOR413 in cold acclimatization protein superfamily, and highly conservative property in species belonged to same genus. By real-time PCR analysis, comparing with southern Camellia species and common varieties, CjCor1 genes had higher expression in C.japonica(Nai Dong) under the condition of low temperature(below 0℃), and also had higher expression in higher cold resistance varieties.

Key words: Camellia japonica(Nai Dong), CjCor1 gene, sequence analysis, gene expression

中图分类号:

S685.14

杨凯, 孙迎坤, 王奎玲, 刘庆华, 高捍东. 耐冬山茶抗寒基因CjCor1的全长克隆与表达分析[J]. 植物研究, 2016, 36(5): 753-759.

YANG Kai, SUN Ying-Kun, WANG Kui-Ling, LIU Qing-Hua, GAO Han-Dong. Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica(Nai Dong)[J]. Bulletin of Botanical Research, 2016, 36(5): 753-759.

参考文献

1. 张乐初,游慕贤,陈德松.中国茶花文化[M].上海:上海文化出版社,2003:85-122.Zhang L C,You M X,Chen D S.Chinese camellia culture[M].Shanghai:Shanghai Culture Publishing House,2003:85-122.
2. 王奎玲.耐冬山茶种质资源研究[D].北京:北京林业大学,2006.Wang K L.Studies on the germplasm resources of Naidong Shancha(Camellia Japonica L.)[D].Beijing:Beijing Forestry University,2006.
3. 黄永会,朱莉莎,王玉臣,等.结球白菜COR基因克隆及序列分析[J].贵州农业科学,2014,42(11):10-13.Huang Y H,Zhu L S,Wang Y C,et al.Gene clone and sequence analysis of Brassica campestris ssp.Pekinensis[J].Guizhou Agricultural Sciences,2014,42(11):10-13.
4. Huang B,Jin L G,Liu H Y.Molecular cloning and functional characterization of a DREB1/CBF-like gene(GhDREB1L) from cotton[J].Science in China Series C:Life Sciences,2007,50(1):7-14.
5. 贺泂杰.高寒冰缘植物高山离子芥CbCBF4基因功能的研究[D].兰州:兰州大学,2012.He J J.The functional research on CbCBF4[D].Lanzhou:Lanzhou University,2012.
6. 洪剑明,邱泽生.植物抗性生理(一)[J].生物学通报,1997(5):13-15.Hong J M,Qiu Z S.Plant resistant physiology(No.1)[J].Bulletin of Biology,1997(5):13-15.
7. 王国莉,郭振飞.植物耐冷性分子机理的研究进展[J].植物学通报,2003,20(6):671-679.Wang G L,Guo Z F.The progress of researches on molecular mechanism of chilling tolerance in plants[J].Chinese Bulletin of Botany,2003,20(6):671-679.
8. 孙迎坤,李纪元,殷恒福,等.重瓣山茶花‘金盘荔枝’C功能基因CjAGL6的全长克隆与表达分析[J].植物研究,2013,33(3):330-338.Sun Y K,Li J Y,Yin H F,et al.Cloning and expression analysis of C function CjAGL6 gene cDNA from Camellia japonica ‘Jinpan Lizhi’[J].Bulletin of Botanical Research,2013,33(3):330-338.
9. 王仁卿,张淑萍,石竹,等.青岛耐冬山茶的多样性特征及其保护[J].山东林业科技,1998,(3):1-4.Wang R Q,Zhang S P,Shi Z,et al.Diversity characteristics and protection of Naidong Shancha in Qingdao[J].Journal of Shandong forestry science and technology,1998(3):1-4.
10. Mikkelsen M D,Thomashow M F.A role for circadian evening elements in cold-regulated gene expression in Arabidopsis[J].The Plant Journal,2009,60(2):328-339.
11. Kilian J,Whitehead D,Horak J,et al.The AtGenExpress global stress expression data set:protocols,evaluation and model data analysis of UV-B light,drought and cold stress responses[J].The Plant Journal,2007,50(2):347-363.
12. Xu W R,Li R M,Zhang N B,et al.Transcriptome profiling of Vitis amurensis,an extremely cold-tolerant Chinese wild Vitis species,reveals candidate genes and events that potentially connected to cold stress[J].Plant Molecular Biology,2014,86(4-5):527-541.
13. 范高韬,孙小明,任小蝶,等.葡萄COR27基因的克隆与抗寒功能研究[J].植物科学学报,2015,33(3):346-354.Fan G T,Sun X M,Ren X D,et al.Cloning and functional analysis of COR27 gene from Vitis vinifera[J].Plant Science Journal,2015,33(3):346-354.
14. 谢素霞,张媛,郭凯琳,等.一个新的茶树黄酮醇合酶基因的克隆和表达分析[J].植物生理学通讯,2009,45(11):1093-1097.Xie S X,Zhang Y,Guo K L,et al.Cloning and expression analysis of a flavonol synthase gene from Camellia sinensis[J].Plant Physiology Communications,2009,45(11):1093-1097.
15. Li X W,Feng Z G,Yang H M,et al.A novel cold-regulated gene from Camellia sinensis,CsCOR1,enhances salt- and dehydration-tolerance in tobacco[J].Biochemical and Biophysical Research Communications,2010,394(2):354-359.
16. Chen L,Zhong H,Ren F,et al.A novel cold-regulated gene,COR25,of Brassica napus is involved in plant response and tolerance to cold stress[J].Plant Cell Reports,2011,30(4):463-471.
17. Seki M,Narusaka M,Abe H,et al.Monitoring the expression pattern of 1300Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray[J].The Plant Cell,2001,13(1):61-72.
18. Breton G,Danyluk J,Charton J B F,et al.Expression profiling and bioinformatic analyses of a novel stress-regulated multispanning transmembrane protein family from cereals and Arabidopsis[J].Plant Physiology,2003,132(1):64-74.
19. 秦华,眭顺照,李名扬,等.蜡梅(Chimonanthus praecox(L.)Link)COR413蛋白基因(Cpcor413pm1)的分子特性与表达分析[J].中国生物化学与分子生物学报,2006,22(7):547-552.Qin H,Sui S Z,Li M Y,et al.Molecular characterization and expression analysis of a novel COR413 gene from Wintersweet (Chimonanthus praecox(L.) Link)[J].Chinese Journal of Biochemistry and Molecular Biology,2006,22(7):547-552.

[1]	徐磊, 胥晓, 刘沁松. 外源水杨酸对盐胁迫下珙桐幼苗抗氧化系统和基因表达的影响[J]. 植物研究, 2023, 43(4): 572-581.
[2]	杨洪, 王立丰, 代龙军, 郭冰冰. 死皮对橡胶树树皮线粒体超微结构及活性氧代谢的影响[J]. 植物研究, 2023, 43(1): 69-75.
[3]	李梦烁, 刘莹泽, 鲁焕, 强胜. 基因转录介导同质园条件下拟南芥不同地理种群的光合能力分化[J]. 植物研究, 2023, 43(1): 90-99.
[4]	覃碧, 刘明洋, 王萌, 王立丰, 黄飞. 橡胶树DELLA基因HbRGL1的克隆与表达分析[J]. 植物研究, 2022, 42(6): 997-1004.
[5]	代龙军, 刘明洋, 阳江华, 周凯, 郭冰冰, 杨洪, 王立丰. 巴西橡胶树胶乳中DUF1262结构域蛋白结构与基因表达分析[J]. 植物研究, 2022, 42(5): 802-810.
[6]	陈思思, 谢牧洪, 崔茂凯, 李文凯, 徐正刚, 贾彩霞, 杨桂燕. 构树转录因子BpbZIP1的鉴定及镉胁迫响应分析[J]. 植物研究, 2022, 42(3): 394-402.
[7]	马凯恒, 何佳凝, 谢牧洪, 任斌, 黄倩雪, 杨桂燕. 核桃JrNPR1基因的克隆与抗病响应潜力[J]. 植物研究, 2022, 42(1): 39-46.
[8]	陈华峰, 樊松乐, 王立丰. 巴西橡胶树赤霉素信号转导途径HbRGA1结构和功能分析[J]. 植物研究, 2021, 41(4): 531-539.
[9]	田红梅, 郭霄, 王奎玲, 孙迎坤. 耐冬山茶子叶再生体系的研究[J]. 植物研究, 2021, 41(3): 321-328.
[10]	柯维忠, 钟雯娟, 刘凯盈, 李祥媛, 尹明华. 黄独微型块茎鲨烯合酶的基因克隆与序列分析[J]. 植物研究, 2021, 41(2): 243-250.
[11]	刘悦, 赵凯, 吕冠斌, 姜廷波, 周博如. 杨树ERF11转录因子基因应答渗透胁迫表达分析[J]. 植物研究, 2020, 40(3): 433-440.
[12]	何好, 朱国庆, 陈诗雅, 徐畅, 金淑梅. 细叶百合LpPEX7基因克隆及盐胁迫下的表达特性分析[J]. 植物研究, 2020, 40(2): 274-283.
[13]	樊松乐, 王纪坤, 谢贵水, 王萌, 王立丰. R2R3型MYB转录因子HbMYB88结构和功能分析[J]. 植物研究, 2020, 40(1): 106-116.
[14]	王琪, 许志茹, 陈瑾元, 张双, 黄佳欢, 刘关君. 杨树重金属相关异戊二烯化植物蛋白(HIPPs)基因的鉴定及表达分析[J]. 植物研究, 2019, 39(6): 935-946.
[15]	张悦, 赵鑫, 侯峥, 王艳敏, 王玉成, 王超. 刚毛柽柳S-腺苷甲硫氨酸合成酶(ThSAMS)基因的克隆及表达分析[J]. 植物研究, 2019, 39(1): 113-122.

耐冬山茶抗寒基因CjCor1的全长克隆与表达分析

Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica(Nai Dong)

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价