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植物研究 ›› 1996, Vol. 16 ›› Issue (1): 114-117.

• 论文 • 上一篇    下一篇

对TMV不同抗性番茄品种叶绿体DNA的RAPD分析

汪清胤1, 傅桂荣1, 刘艳霞1, 黄永芬1, 郑洪刚2, 谢纬武2, 王斌2   

  1. 1. 哈尔滨师范大学生物系, 黑龙江, 哈尔滨 150080;
    2. 中科院遗传研究所, 北京 100101
  • 收稿日期:1995-05-01 出版日期:1996-03-15 发布日期:2016-06-14
  • 基金资助:
    国家自然科学基金资助项目。

THE ANALYSIS OF ct-DNA RESISTANT AND SENSITIVE TO TMV WITH RAPD IN TOMATO VARIETIES

Wang Qing-yin1, Fu Gui-rong1, Liu Yan-xia1, Huang Yong-fen1, Zhang Hong-gang2, Xie Wei-wu2, Wang Bin2   

  1. 1. Biology Department of Harbin Normal University Harbin, 150080, Heilongjiang;
    2. Institute of Genetics, Academia Sinica, Beijing, 100101
  • Received:1995-05-01 Online:1996-03-15 Published:2016-06-14

摘要: 本实验对TMV抗性和敏感的两个番茄品种ct-DNA进行RAPD分析,结果发现在20个引物中有4个引物的扩增产物存在明显差异,共显示出14条差异带。这可能是由于ct-DNA碱基顺序变异或小片段DNA插入或缺失造成的,深入研究将对探讨番茄抗TMV的分子机理,进而用遗传工程方法培育抗病品种具有重要意义。

关键词: 番茄, 叶绿体DNA, TMV, RAPD

Abstract: In the present study, we analyse the ct-DNA of tomato which is resistant and sensitive to TMV with RAPD. We find difference in four of the twenty primers. 14 different bands are showed. We guess this maybe alternation of base sequence or insertion or deletion of short DNA sequence. It will play an important role in study of machinism of TMV resistance and construction of virus resistant tomato.

Key words: Tomato, ct_DNA, TMV, RAPD.