欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2012, Vol. 32 ›› Issue (4): 484-487.doi: 10.7525/j.issn.1673-5102.2012.04.018

• 论文 • 上一篇    下一篇

濒危药用植物桃儿七的离体培养研究

郭琪1,2;张军1,2;赵小锋1,2;杨涛1,2;王沛雅1,2;杨晖1,2*   

  1. 1.甘肃省科学院生物研究所,兰州 730000;2.甘肃省工业微生物工程技术研究中心,兰州 730000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-20 发布日期:2012-07-20
  • 通讯作者: 杨晖
  • 基金资助:
     

In vitro Culture of Endangered Medicinal Plant Sinopodophyllum hexandrum Royle

GUO Qi;ZHANG Jun;ZHAO Xiao-Feng;YANG Tao;WANG Pei-Ya;YANG Hui;*   

  1. 1.Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000;2.Industrial Microbiology Engineering Research Center in Gansu,Lanzhou 730000
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-20 Published:2012-07-20
  • Contact: YANG Hui
  • Supported by:
     

摘要: 以桃儿七种子诱导的无菌苗为材料,研究了外源激素对芽诱导、增殖和生根的影响,建立了桃儿七离体培养再生体系。结果表明:芽诱导阶段,采用3.0 mg·L-1 6-BA+0.5 mg·L-1 GA3激素组合,出芽率最高可达85-71%,缩短出芽时间30~40 d,在添加3.0 mg·L-1 6-BA+0.2 mg·L-1 IAA的MS培养基上利于增殖,增殖速度快,增殖系数1.63。以WPM+1.5 mg·L-1 IAA+0.5 mg·L-1 NAA的培养基培养30 d 后,生根率可达60.1%。

关键词: 桃儿七, 无菌苗, 激素, 离体培养

Abstract: The seeds of Sinopodophyllum hexandrum Royle were taken as materials. The effects of exogenous hormones on induction, multiplication and rooting of explants were discussed. The regeneration system of S.hexandrum has been established. The results showed that: The optimal conditions for shoot induction were 3.0 mg·L-1 6-BA+0.5 mg·L-1 GA-3. The induction rate was 85.71% with a shortening of 30-40 d. For multiplication, the optimal conditions were 3.0 mg·L-1 6-BA+0.2 mg·L-1 IAA, and the mean shoots obtained per explant was 1.63. The most proper rooting medium was: WPM+1.5 mg·L-1 IAA+0.5 mg·L-1 NAA and the rooting ratio was 60.1% after 30 d culturing.

Key words: Sinopodophyllum hexandrum Royle, seeding, hormones, In vitro culture

中图分类号: