不同时间下低温胁迫对油棕幼苗生理生化变化的影响

doi:10.7525/j.issn.1673-5102.2015.06.012

摘要/Abstract

摘要： 为了研究不同时间的低温胁迫对油棕幼苗生理生化变化的影响，以8个月苗龄的油棕幼苗为材料，根据海南的气温变化规律，设置4个不同的最低温度处理，分别为28℃(CK处理)，18℃（T1处理）、12℃（T2处理）、6℃（T3处理），分别处理0、7、14、21 d时取样。结果发现，随着温度的下降和低温胁迫时间的延长，幼苗叶片含水量、SOD活性不断下降；可溶性蛋白、可溶性糖含量、H₂O₂、MDA含量不断升高；POD、CAT、APX活性先上升而后下降；T2、T3处理2周后的幼苗其心叶都变褐色，且生长明显减缓。低温会对油棕幼苗造成了明显地氧化伤害，但油棕幼苗可能通过提高可溶性蛋白、可溶性糖等物质的含量，抗氧化酶维持一定活性的适应机制，以抵抗低温造成的氧化伤害，维持其正常生长。

关键词: 油棕, 低温, MDA, 抗氧化酶

Abstract:

We used eight months of oil palm seedlings as materials to study the effect of different time of low temperature stress on physiological and biochemical changes of oil palm seedling, and according to the temperature variation in Hainan, set up four different minimum temperature treatments including CK(natural temperature treatment), 18℃(T1 treatment), 12℃(T2 treatment) and 6℃(T3 treatment) after 0, 7, 14, 21 d sampling, respectively. With the decreasing of temperature and the increasing of time, the oil palm seedling leaf water content and SOD activity continued to decrease, the soluble protein, sugar content, H₂O₂ and MDA content continued to increase, and POD, CAT, APX activity first increased and then decreased. Under T2, T3 the seedling leaf became brown and oil palm seedling growth slowed down significantly after twoweek treatment. Low temperature caused obviously oxidative damage, but oil palm seedlings used adaptation mechanisms by increasing the contents of soluble protein, sugar and antioxidant enzyme activity to resist oxidative damage caused by low temperature for normal growth.

Key words: oil palm, low temperature, MDA, antioxidase

中图分类号:

刘艳菊1,2；曹红星1,2*；张如莲3. 不同时间下低温胁迫对油棕幼苗生理生化变化的影响[J]. 植物研究, 2015, 35(6): 860-865.

LIU Yan-Ju1,2；CAO Hong-Xing1,2*；ZHANG Ru-Lian3. Effect of Low Temperature Stress on Physiological Changes in Oil Palm(Elaesis guineensis Jacg.) Seedling under Different Time[J]. Bulletin of Botanical Research, 2015, 35(6): 860-865.

参考文献

1.Sue T T,Pantzaris T P.Pocketbook of palm oil uses［J］.Malaysia Palm Oil Board(MPOB),2010:3-7.

2.雷新涛,曹红星.油棕［M］.北京:中国农业出版社,2013:31-36.

3.杨华庚.低温胁迫对油棕幼苗生理生化特性的影响［D］.儋州:华南热带农业大学,2007.

4.曹红星,孙程旭,冯美利,等.低温胁迫对海南本地种油棕幼苗的生理生化响应［J］.西南农业学报,2011,24(4):128 2-1285.

5.李静,陈秀龙,李志阳,等.低温胁迫对10个油棕新品种生理生化特性的影响［J］.华南农业大学学报,2013,34(1):62-66.

6.黄秉维.竺可桢同志与我国热带和海南岛的科学研究(一)我国热带、亚热带界线问题［J］.地理研究,1984,3(1):8-18.

7.林良勋,吴乃康,蔡安安,等.广东2008年低温雨雪冰冻灾害及气象应急响应［J］.气象,2009,35(5):26-33.

8.李合生.植物生理生化实验原理和技术［M］.北京:高等教育出版社,2006.

9.Patterson B D,Macrae E A,Ferguson I B.Estimation of hydrogen peroxide in plant extracts using titanium(Ⅳ)［J］.Anal Biochem,1984,139:487-492.

10.张志良,瞿伟菁.植物生理学实验指导［M］.北京:高等教育出版社,2003:274-276.

11.Bai T,Li C,Ma F,et al.Responses of growth and antioxidant system to root zone hypoxia stress in two Malus species［J］.Plant Soil,2009,327(1-2):95-105.

12.Rathmell W G,Sequeira L.Soluble peroxidase in fluid from the intercellular spaces of tobacco leaves［J］.Plant Physiology,1974,53:317-318.

13.Aebi H.Catalase in vitro［J］.Methods in Enzymology,1984,105:121-126.

14.Ma F H,Cheng L L.The sun-exposed peel of apple fruit has higher xanthophyll cycle dependent thermal dissipation and antioxid-ants of the ascorbate glutathione pathway than the shaded peel［J］.Plant Science,2003,165:819-827.

15.Kang H M,Saltveit M E.Activity of enzymatic antioxidant defence systems in chilled and heat shocked cucumber seedling radicles［J］.Plant Physiol,2001,113:548-556.

16.Orvar B L,Sangwan V,Omann F,et al.Early steps in cold sensing by plant cells:The role of actin cytoskeleton and membrane fluidity［J］.Plant J,2000,23:785-794.

17.Arbaoui M,Balko C,Link W.Study of faba bean(Vicia faba L.) winter-hardiness and development of screening methods［J］.Field Crops Research,2008,106:60-67.

18.曹建华,李静,陶忠良,等.油棕幼苗对低温胁迫的生理响应及其抗寒力评价［J］.热带农业科学,2014(8):8-12.

19.Ma Y Y,Zhang Y L,Shao H,et al.Differential physio-biochemical responses to cold stress of cold-tolerant and non-tolerant grapes(Vitis) from China［J］.Journal of Agronomy and Crop Science,2010,196(3):212-219.

20.李合生.现代植物生理学［M］.北京:高等教育出版社,2002:389-396.

21.Sagi M,Fluhr R.Production of reactive oxygen species by plant NADPH oxidases［J］.Plant Physiology,2006,141(2):336-340.

22.Skopelitis D S,Paranychianakis N V,Paschalidis K A.Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine［J］.The Plant Cell,2006,18(10):2767-2781.

23.Prasad T K.Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings:changes in antioxidant system,oxidation of proteins and lipids,and protease activities［J］.The Plant Journal,1996,10(6):1017-1026.

24.倪书邦,刘世红,魏丽萍,等.西双版纳新引油棕品种抗寒性鉴定及抗氧化系统研究［J］.云南农业大学学报,2012,27(1):44-48.

25.Lee S H,Singh A P,Chung G C.Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decreases in water transport［J］.Journal of experimental botany,2004,55(403):1733-1741.

26.郭卫丽.辣椒对低温胁迫的响应与其低温抗性相关基因的克隆和功能分析［D］.杨凌:西北农林科技大学,2013.

27.刘泓,叶媛蓓,崔波,等.多环芳烃荧蒽诱导拟南芥氧化胁迫［J］.应用生态学报,2008,19(2):413-418.

28.Lin C C,Kao C H.Effect of NaCl stress on H₂O₂ metabolism in rice leaves［J］.Plant Growth Regulation,2000,30(2):151-155.

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