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植物研究 ›› 2010, Vol. 30 ›› Issue (6): 718-724.doi: 10.7525/j.issn.1673-5102.2010.06.017

• 论文 • 上一篇    下一篇

3个三角梅品种中类黄酮的毛细管电泳分析比较研究

徐夙侠1;林春松1;黄青云1;LI Li2;曾碧玉1   

  1. 1.福建省亚热带植物研究所,厦门 361006;2.Plant,Soil and Nutrition Laboratory,Department of Plant Breeding and Genetics,Cornell University,Ithaca,NY 14853
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
  • 基金资助:
     

Analysis and Comparison of Flavonoids in Three Bougainvilleas by Micellar Electrokinetic Chromatography

XU Su-Xia;LIN Chun-Song;HUANG Qing-Yun;LI Li;ZENG Bi-Yu   

  1. 1.Key Laboratory of Physiology and Biochemistry,Fujian Institute of Subtropical Botany,780-800 Jiahe Road,Xiamen 361006;2.Plant, Soil and Nutrition Laboratory,Department of Plant Breeding and Genetics,Cornell University,Ithaca,NY 14853
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
  • Supported by:
     

摘要: 甜菜色素和属于类黄酮的花青素在自然界排斥分布,然而我们在甜菜色素代谢类型植物三角梅中发现积累多种类黄酮。为了进一步证实这一现象,本研究对3种白花三角梅(Bougainvillea sp.,Bougainvillea glabra‘Alba’,and Bougainvillea‘White Stripe’)中的5种类黄酮(槲皮素、山奈酚、异鼠李素、杨梅素和芹菜素)采用毛细管电泳分析方法进行了调查。结果表明,只有3种类黄酮:槲皮素、山奈酚、异鼠李素被检测出。3个材料中,被探测出来的总黄酮含量最高的是Bougainvillea glabra‘Alba’,1 749 μg·g-1Bougainvillea‘White Stripe’ 1 241 μg·g-1以及B.sp1 312 μg·g-1。被探测出来的3种黄酮中,槲皮素含量最高852~1 114 μg·g-1,异鼠李素161~628 μg·g-1,而山奈酚之被检测出微量。本研究对于定性定量可食用的三角梅中的类黄酮组成是十分有意义的。甜菜色素和类黄酮在三角梅中的积累关系以及可能的功能本研究也做了讨论。

关键词: 三角梅, 槲皮素, 山奈酚, 异鼠李素, 类黄酮, MEKC

Abstract: The exclusion distribution of betalain and flavonoid-derived anthocyanins and existing of flavonoids in betalain-plants led to our research. Quercetin, kaempferol, myricetin, apigenin, and isorhamnetin were simultaneously detected in three white varieties of Bougainvillea(Bougainvillea sp., Bougainvillea glabra ‘Alba’, and Bougainvillea ‘White Stripe’) for the first time, using micellar electrokinetic chromatography (MEKC). Among the five flavonoids, the three flavonols were detected, and one flavone and one flavonol were not. The total content of the three flavonoids were 1 749 μg·g-1 in B.glabra ‘Alba’, 1 241 μg·g-1 in B. ‘White Stripe’ and 1 312 μg·g-1 in B. sp. In the detected flavonoids, the content of quercetin was the highest and ranged from 852-1 114 μg·g-1, isorhamnetin varied from 161-628 μg·g-1 in three species. Kaempferol was detected at trace amounts. This work is valuable to identify and quantify the naturally occurring flavonoids in the edible bracts of three white varieties of Bougainvillea. The relationship between betalain and flavonoids in bougainvilleas and possible functions were discussed.

Key words: Flavonoids, quercetin, kaempferol, isorhamnetin, Bougainvilleas, MEKC

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