转基因金叶银中杨叶色及生长变异分析

doi:10.7525/j.issn.1673-5102.2020.06.012

摘要/Abstract

摘要：

转PaGLK基因银中杨抑制表达株系叶绿素含量显著降低，叶片呈现黄色（命名为金叶银中杨），以转PaGLK基因的银中杨为材料，测定其叶色参数和叶绿素含量的时序变化规律、分析生长特性。结果显示，转PaGLK基因的银中杨使叶片颜色发生改变，抑制表达株系整个生长期叶绿素含量显著低于WT（P<0.05），叶色亮度显著高于WT（P<0.05），并且在生长发育期叶片一直呈现深黄绿色。抑制表达株系中的Y2速生期内苗高日生长量（GD）高于对照株系，苗期株高不受影响。而过表达转基因银中杨的当年高生长都显著低于对照株系（P<0.05），其速生期内苗高日生长量均值（GD）也低于对照株系，其均值为对照株系的22.19%。PaGLK抑制表达株系在城市园林绿化具有潜在的应用价值。

关键词: 银中杨, PaGLK, 叶绿素含量, 生长特性

Abstract:

The chlorophyll content of the PaGLK gene Populus alba×P.berolinensis inhibited expression line was significantly reduced， and the leaves showed yellow（named golden leaf silver poplar）. Using PaGLK transgenic Populus alba×P.berolinensis， its leaf color parameters and chlorophyll content were measured in time series， and its growth characteristics were analyzed. The results showed that PaGLK transgenic Populus alba×P.berolinensis changed the leaf color， and the chlorophyll content of the expressing lines was significantly lower than that of WT（P<0.05）， and the leaf brightness was significantly higher than that of WT（P<0.05）. During the growth and development period， the leaves showed dark yellow green. The daily growth height（GD） of seedling height in the Y2 fast-growing stage of the expressing lines was higher than that of the control lines， and the plant height at the seedling stage was not affected. The over-expressing transgenic Populus alba×P.berolinensis plants were significantly lower than the control plants in that year（P<0.05）， and the average daily growth height（GD） of the seedling height in the fast-growing period was also 22.19% of that of the control plants. PaGLK inhibited expression strains have potential application value in urban landscaping.

Key words: Populus alba×P. berolinensis, PaGLK, chlorophyll content, growth characteristics

中图分类号:

Q943

李艺迪, 顾宸瑞, 冮慧欣, 刘桂丰, 陈肃, 姜静. 转基因金叶银中杨叶色及生长变异分析[J]. 植物研究, 2020, 40(6): 897-905.

Yi-Di LI, Chen-Rui GU, Hui-Xin GANG, Gui-Feng LIU, Su CHEN, Jing JIANG. Leaf Color and Growth Variation of Transgenic Gold Leaf Poplar[J]. Bulletin of Botanical Research, 2020, 40(6): 897-905.

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引物 Primer	序列 Sequence(5′—3′)
PaGLK-F	ATGCAAAACACTGCAACCAC
PaGLK-R	TCACGTTGGTGGTATCTTTGGAACT
p5941-Cis-F	ATAAGGAAGTTCATTTCATTTG
p5941-Cis-R	CAATCAAATGAAGAGCCAAT
p5941-Anti-F	CTTACTTACACTTGCCTTGGAG
p5941-Anti-R	ATCTGAGCTACACATGCTCAG

引物 Primer	序列 Sequence(5′—3′)
PaGLK-F	CCCATGCACCACCACTTTAG
PaGLK-R	GTCCGGTAAGGAGGCCAATG
Psn18S-F	CCGTCCTAGTCTCAACCATA
Psn18S-R	CTCTCAGTCTGTCAATCCTTAC

株系 strain	封顶后苗高实测值 Measure values of height when test lines stop growing(cm)	R²	苗木开始生长时苗高值a Values of height when test lines start growing(cm)	苗木停止生长时苗高值b Values of height when test lines stop growing(cm)	速生点t₀ Fast-growing point(d)	苗高速生点处生长速度 The growing speed on fast-growing point(cm?d^-1)
WT	138.7a	0.997 3	16a	138.7a	182	2.135
G1	103.9c	0.997 2	5.4e	103.9c	185	1.650
G2	125.9b	0.997 0	10.2cd	125.9b	187	1.793
G3	110.5c	0.996 4	9.07d	110.5c	189	1.556
Y1	129.2ab	0.995 6	12bc	129.2ab	182	2.025
Y2	137.5a	0.996 5	11.1c	137.5a	182	2.235
Y3	131ab	0.997 7	13.1b	131ab	188	1.838

株系 Strain	速生点t₀ Fast-growing point(d)	持续时间RR Lasting time (d)	生长期参数 Parameters in the growing stage
株系 Strain	速生点t₀ Fast-growing point(d)	持续时间RR Lasting time (d)	GR (cm)	GD (cm?d^-1)	RRA (%)
WT	182	40	74.817	1.870	57.3
G1	185	42	60.694	1.445	57.4
G2	187	47	73.561	1.565	57.8
G3	189	48	65.079	1.356	58.2
Y1	182	41	72.516	1.769	57.6
Y2	182	39	76.506	1.962	57.1
Y3	188	47	75.362	1.603	58.0

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