金黄壳囊孢菌侵染过程中银中杨锌指蛋白基因的表达

doi:10.7525/j.issn.1673-5102.2014.04.014

植物研究 ›› 2014, Vol. 34 ›› Issue (4): 510-515.doi: 10.7525/j.issn.1673-5102.2014.04.014

金黄壳囊孢菌侵染过程中银中杨锌指蛋白基因的表达

杨明秀^1,2；宋瑞清^1*

1.东北林业大学林学院，哈尔滨　150040；2.东北农业大学农学院，哈尔滨　150030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-07-20 发布日期:2014-07-20
通讯作者: 宋瑞清
基金资助:

Expression of Zinc Finger Protein Gene in Populus alba×P.berolinensis in the Infection Process by Cytospora chrysosperma

YANG Ming-Xiu;SONG Rui-Qing*

1.Forestry School,Northeast Forestry University,Harbin　150040；2.Agricultural School,Northeast Agricultural University,Harbin　150030

Received:1900-01-01 Revised:1900-01-01 Online:2014-07-20 Published:2014-07-20
Contact: SONG Rui-Qing
Supported by:

摘要/Abstract

摘要： 研究了银中杨接种金黄壳囊孢菌后锌指蛋白基因的表达，为林木抗病遗传育种工作奠定基础。从杨树上分离得到了一株杨树烂皮病菌（CHH001），测定其对银中杨的致病力，并采用形态学和ITS-PCR鉴定方法鉴定该菌株为金黄壳囊孢菌。采用实时定量荧光PCR技术对银中杨接种金黄壳囊孢菌后锌指蛋白基因的表达进行了研究。实验结果显示，ZFP1和69-Ⅳ-2-4在接种后表达水平持续上升，ZFP1在48 h的基因表达量分别为烫伤对照和空白对照的4.21倍和3.11倍；69-Ⅳ-2-4在48 h为烫伤对照和空白对照的9.56和7.06倍。ZFP1和69-Ⅳ-2-4在接种前期高表达，表明可能参与了植物与病原物的识别过程和防御反应。

关键词: 银中杨, 金黄壳囊孢菌, 锌指蛋白基因, 实时定量RT-PCR

Abstract: The expression of zinc finger protein gene after inoculation by Cytospora chrysosperma was studied, which lays foundation for poplar disease-resistant breeding. The virulence of a bacterial strain (CHH001) isolated from poplar to the P.alba×P.berolinensis was determined by inoculation. The bacterial strain was identified as C.chrysosperma using the methods of morphology and ITS-PCR. Real time RT-PCR was used to study the expression of resistance genes of P.alba×P.berolinensis in this study. ZFP1 and 69-Ⅳ-2-4 have higher levels of relative expression. The results showed that the expressions of ZFP1 and 69-Ⅳ-2-4 continuously increased after inoculation. The expressions of ZFP1 were 4.21 times and 3.11 times of the scalded control and blank control, respectively, after inoculation for 48 h. The expressions of 69-Ⅳ-2-4 were 9.56 times and 7.06 times of the scalded control and blank control, respectively, after inoculation for 48 h. The expressions of ZFP1 and 69-Ⅳ-2-4 were higher in early inoculation. It showed that the zinc finger protein gene may participate in the processes of plant-pathogen recognition and defense.

Key words: Populus alba×, P.berolinensis, Cytospora chrysosperma, zinc finger protein gene, real time RT-PCR

中图分类号:

Q786

杨明秀;宋瑞清*. 金黄壳囊孢菌侵染过程中银中杨锌指蛋白基因的表达[J]. 植物研究, 2014, 34(4): 510-515.

YANG Ming-Xiu;SONG Rui-Qing*. Expression of Zinc Finger Protein Gene in Populus alba×P.berolinensis in the Infection Process by Cytospora chrysosperma[J]. Bulletin of Botanical Research, 2014, 34(4): 510-515.

金黄壳囊孢菌侵染过程中银中杨锌指蛋白基因的表达

Expression of Zinc Finger Protein Gene in Populus alba×P.berolinensis in the Infection Process by Cytospora chrysosperma

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