木霉菌对芸芥生长相关生理指标的影响

doi:10.7525/j.issn.1673-5102.2020.03.004

植物研究 ›› 2020, Vol. 40 ›› Issue (3): 347-352.doi: 10.7525/j.issn.1673-5102.2020.03.004

木霉菌对芸芥生长相关生理指标的影响

侯雪月¹, 邓俊杰¹, 姚志红¹, 黄寿臣¹, 李嘉哲¹, 茶新有¹, 马德志², 张荣沭¹

1. 东北林业大学园林学院, 哈尔滨 150040;
2. 齐齐哈尔医学院黄芪产业研究院, 齐齐哈尔 161000

收稿日期:2019-12-27 出版日期:2020-05-05 发布日期:2020-05-29
通讯作者: 张荣沭,E-mail:zrs6504@nefu.edu.cn E-mail:zrs6504@nefu.edu.cn
作者简介:侯雪月(1996-),女,硕士研究生,主要从事园林植物种质资源研究及植物病害生物防治。
基金资助:
国家自然科学基金资助项目（31370642）；黑龙江省自然科学基金联合引导项目（LH2019C015）

Effects of Trichoderma on the Growth-related Physiological Indexes of Rocketsalad(Eruca sativa)

HOU Xue-Yue¹, DENG Jun-Jie¹, YAO Zhi-Hong¹, HUANG Shou-Chen¹, LI Jia-Zhe¹, CHA Xin-You¹, MA De-Zhi², ZHANG Rong-Shu¹

1. College of Landscape Architecture, Northeast Forestry University, Harbin 150040;
2. Institute of Astragalus Industry, Qiqihar Medical University, Qiqihar 161000

Received:2019-12-27 Online:2020-05-05 Published:2020-05-29
Supported by:
National Natural Science Foundation of China(31370642);Joint Guidance(Lian-He-Yin-Dao) project of the Natural Science Foundation of Heilongjiang Province(LH2019C015)

摘要/Abstract

摘要： 芸芥（Eruca sativa）是当今我国蔬菜市场中一种颇具开发价值的新特芳香蔬菜。为了研制促进芸芥生长和提高品质的生防木霉菌剂，本研究以芸芥为试材，在大田条件下，采用本实验室分离鉴定的哈茨木霉T8进行浸种和浇根处理，分析木霉菌对一个生长季内连续栽培三茬30 d龄芸芥的生长相关生理指标的影响。结果表明，施用木霉菌能显著提高芸芥的生物量，改善其光合特性，增强其防御酶活性和脯氨酸含量，提高其产量和营养价值。

关键词: 哈茨木霉T8, 芸芥, 生物量, 品质, 抗氧化活性

Abstract: Rocketsalad(Eruca sativa) is a kind of new special and aromatic vegetable with great development value in China's vegetable market. In order to develop biocontrol agents of Trichoderma to promote the growth and quality of rocketsalad, the seed soaking and root-watering treatment of rocketsalad with T.harzianum T8 which was isolated and identified in our laboratory were carried out. And the effects of Trichoderma on the growth-related physiological indexes of rocketsalad aged 30 d were analyzed in continuous cultivation of three stubble in a vegetation season. The results showed that the application of Trichoderma can significantly increase the biomass of rocketsalad, improve the photosynthetic characteristics, enhance the resistance-related enzyme activity and proline content, and raise the yield and the nutritional value.

Key words: Trichoderma harzianum T8, rocketsalad, biomass, quality, antioxidant activity

中图分类号:

Q949.748.3

侯雪月, 邓俊杰, 姚志红, 黄寿臣, 李嘉哲, 茶新有, 马德志, 张荣沭. 木霉菌对芸芥生长相关生理指标的影响[J]. 植物研究, 2020, 40(3): 347-352.

HOU Xue-Yue, DENG Jun-Jie, YAO Zhi-Hong, HUANG Shou-Chen, LI Jia-Zhe, CHA Xin-You, MA De-Zhi, ZHANG Rong-Shu. Effects of Trichoderma on the Growth-related Physiological Indexes of Rocketsalad(Eruca sativa)[J]. Bulletin of Botanical Research, 2020, 40(3): 347-352.

参考文献

[1] 寇凤仙,樊新华,齐巧丽.芝麻菜的开发利用及栽培技术[J].河北农业科技,2002,(8):14.Kou F X,Fan X H,Qi Q L,et al.Development,utilization and cultivation techniques of rocketsalad[J].Hebei Agricultural Science and Technology,2002,(8):14.
[2] 冯均科.芳香保健特菜芝麻菜栽培技术[J].上海农业科技,2007,(1):80.Feng J K.Cultivation techniques of aromatic health specialty vegetables-rocketsalad[J].Shanghai Agricultural Science and Technology,2007,(1):80.
[3] Bae S J,Mohanta T K,Chung J Y,et al.Trichoderma metabolites as biological control agents against Phytophthora pathogens[J].Biological Control,2016,92:128-138.
[4] Harman G E,Howell C R,Viterbo A,et al.Trichoderma species-Opportunistic,avirulent plant symbionts[J].Nature Reviews Microbiology,2004,2(1):43-56.
[5] 伍晓丽,谭均,崔广林,等.深绿木霉对青蒿的促生作用[J].西南大学学报:自然科学版,2019,41(7):36-42.Wu X L,Tan J,Cui G L,et al.Promoting effects of Trichoderma atroviride on Artemisia annual growth[J].Journal of Southwest University:Natural Science Edition,2019,41(7):36-42.
[6] 薛应钰,叶巍,杨树,等.高效溶磷木霉菌株复合诱变选育及促生作用[J].核农学报,2018,32(12):2289-2297.Xue Y Y,Ye W,Yang S,et al.Growth-promoting effect and screening of high phosphorus dissolving strains of Trichoderma sp.by compound mutation[J].Journal of Nuclear Agricultural Sciences,2018,32(12):2289-2297.
[7] 朱双杰.哈兹木霉对植物的促生作用及其机制研究[D].合肥:安徽农业大学,2007.Zhu S J.On the effects and the mechanisms of growth promoting of Trichoderma harzianum to plant[D].Hefei:Anhui Agricultural University,2007.
[8] Lian L,Wang X W,Zhu Y S,et al.Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene[J].Molecular Biology Reports,2014,41(4):2189-2197.
[9] 许大全.光合作用效率[M].上海:上海科学技术出版社,2002:163-167.Xu D Q.The efficiency of photosynthesis[M].Shanghai:Shanghai Scientific & Technical Publishers,2002:163-167.
[10] 陈伯清,屈海泳,刘连妹.木霉HT-03对番茄幼苗叶绿素和保护酶的影响[J].江苏农业科学,2007,(3):112-114.Chen B Q,Qu H Y,Liu L M.Effects of Trichoderma harziarum strain HT-03 on chlorophyl content and antioxidases activity of tomato seedling[J].Jiangsu Agricultural Sciences,2007,(3):112-114.
[11] Siddiqui Y,Meon S,Ismail R,et al.Bio-efficiency of compost extracts on the wet rot incidence,morphological and physiological growth of okra(Abelmoschus esculentus (L.) Moench)[J].Scientia Horticulturae,2008,117(1):9-14.
[12] 王慧,刘志华,吕曼曼,等.棘孢木霉菌对山新杨移栽苗叶片光合特性的影响[J].北方园艺,2013(23):78-82.Wang H,Liu Z H,Lü M M,et al.Effects of Trichoderma asperellum on leaf photosynthesis of tissue-cultured Populus davidiana×P.bolleana Loucne seedlings transplanted into pots[J].Northern Horticulture,2013,(23):78-82.
[13] 杨雄,刘乐平,李兴山,等.哈茨木霉对湿地松幼苗光合作用和生物量累积的影响[J].湖北林业科技,2018,47(6):27-30.Yang X,Liu L P,Li X S,et al.Effects of Trichoderma harzianum infection on photosynthesis rates and biomass accumulation of seedlings of Pinus elliottii[J].Hubei Forestry Science and Technology,2018,47(6):27-30.
[14] Fu J,Wang Y F,Liu Z H,et al.Trichoderma asperellum alleviates the effects of saline-alkaline stress on maize seedlings via the regulation of photosynthesis and nitrogen metabolism[J].Plant Growth Regulation,2018,85(3):363-374.
[15] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:137-202.Gao J F.Experimental guidance for plant physiology[M].Beijing:Higher Education Press,2006:137-202.
[16] 刘志洋.游离脯氨酸含量测定法比较宿根花卉抗寒性[J].黑龙江农业科学,2016(11):102-105.Liu Z Y.Cold resistance comparison of perennial flowers by determination of free Proline content[J].Heilongjiang Agricultural Sciences,2016(11):102-105.
[17] 王学奎.植物生理生化实验原理和技术:2版[M].北京:高等教育出版社,2006:278-279.Wang X K.Principles and techniques of plant physiological biochemical experiment:2nd ed[M].Beijing:Higher Education Press,2006:278-279.
[18] 张志良.植物生理学实验指导[M].北京:高等教育出版社,2001:35-36.Zhang Z L.Guidance on plant physiology experiment[M].Beijing:Higher Education Press,2001:35-36.
[19] 李振国.现代植物生理学实验指南[M].北京:科学出版社,1999:302-303.Li Z G.Guide to modern plant physiology experiments[M].Beijing:Science Press,1999:302-303.
[20] 刘洪竹,赵习姮,陈双颖,等.热激处理对鲜切甜椒活性氧代谢及贮藏品质的影响[J].食品工业科技,2014,35(1):310-314.Liu H Z,Zhao X H,Chen S Y,et al.Effect of heat shock treatment on reactive oxygen species metabolism and storage quality of fresh-cut sweet pepper[J].Science and Technology of Food Industry,2014,35(1):310-314.
[21] 李成忠,孙燕,杜庆平.黄连木根系抗氧化酶系对干旱胁迫的响应[J].林业科技开发,2008,22(2):57-60.Li C Z,Sun Y,Du Q P,et al.Responses of anti-oxidative enzymes in Pistacia chinensis roots to drought stress[J].China Forestry Science and Technology,2008,22(2):57-60.
[22] Takahama U,Oniki T.A peroxidase/phenolics/ascorbate system can scavenge hydrogen peroxide in plant cells[J].Physiologia Plantarum,1997,101(4):845-852.
[23] 韩冬梅,吴振先,季作梁,等.SO₂对龙眼果实的氧化作用与衰老的影响[J].果树科学,1999,16(1):24-29.Han D M,Wu Z X,Ji Z L,et al.Effects of SO₂ treatment on the oxidation and senescence of Longan fruit[J].Journal of Fruit Science,1999,16(1):24-29.
[24] 严俊鑫,关桦楠,迟德富,等.机械损伤对重瓣玫瑰防御酶的诱导[J].植物研究,2013,33(4):499-503,507.Yan J X,Guan H N,Chi D F,et al.Induction of defensive enzymes in leaves of Rosa rugosa ‘plena’ by mechanical wounding[J].Bulletin of Botanical Research,2013,33(4):499-503,507.
[25] 廉华,姜海洋,张东雪,等.不同采收时期对芝麻菜营养品质和相关生理指标的影响[J].中国土壤与肥料,2014,(5):75-79.Lian H,Jiang H Y,Zhang D X,et al.Effects of different harvest time on nutrient quality and relative physiological indexes of Eruca sativa Mill[J].Soils and Fertilizers Sciences in China,2014,(5):75-79.
[26] 关锦毅,郝再彬,张达,等.叶绿素提取与检测及生物学功效的研究进展[J].东北农业大学学报,2009,40(12):130-134.Guan J Y,Hao Z B,Zhang D,et al.A review on the extraction,detection and biological function of chlorophyll[J].Journal of Northeast Agricultural University,2009,40(12):130-134.

木霉菌对芸芥生长相关生理指标的影响

Effects of Trichoderma on the Growth-related Physiological Indexes of Rocketsalad(Eruca sativa)

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