连香树人工林根系分泌物输入季节性变化及其驱动的根际微生物特性研究

doi:10.7525/j.issn.1673-5102.2018.01.006

摘要/Abstract

摘要： 根系分泌物介导的根际过程具有重要的生态学效应，但目前有关植物尤其是濒危珍稀植物根系分泌物及其介导的土壤生物地球化学循环过程的原位季节动态研究甚少。本文以阿坝州茂县大沟流域30年生的国家二级濒危保护植物连香树人工林为研究对象，于2014年4、7、9和12月利用原位收集装置对连香树根系分泌物进行原位收集；并同步分析了根际土壤微生物特性的变化。结果表明：（1）根系分泌物C和N分泌速率均呈现明显的季节动态变化，其中，夏季最高，冬季最低。（2）根际土壤微生物碳、氮及土壤酶活性显著高于非根际，表现出正的根际效应；根际效应也与根系分泌物输入表现出类似的季节动态规律，即夏季（7月）最高，而冬季（12月）最低。（3）进一步相关分析表明：根系分泌物分泌速率与土壤微生物碳、氮及土壤酶活性根际效应值呈线性正相关，表明根系分泌物输入是驱动根际微生物活性的重要因子，揭示连香树根系碳输入对根际土壤过程和功能的季节响应。未来研究应加强根系分泌物输入与土壤生物地球化学循环过程的偶联效应与机制研究。

关键词: 连香树人工林, 根系分泌物, 根际微生物, 土壤酶活性, 季节变化

Abstract: The rhizosphere processes involved in exudate have important ecological effects, but there is very little seasonal changes in situ of plants root exudatuions and their mediated soil biogeochemical cycles, especially endangered rare plant. With 30-year Cercidiphyllum japonicum Sieb.. plantation which is national secondary endangered plants in Dagou in west Sichuan, we used the in situ improved collecting device to study C.japonicum Sieb. root exudates in April, July, September and December 2014, collect rhizosphere and non-rhizosphere soil, and synchronously analyze the changes of rhizosphere microbiological properties. The results showed that:(1)the root secretion of dissolve organic carbon(DOC), total nitrogen(TN) rate appeared remarkable seasonal changes, the highest in summer and lowest in winter. (2)Rhizosphere of microbial biomass carbon(MBC), microbial biomass nitrogen(MBN) was significantly higher non-rhizosphere soil that showed the positive rhizosphere effect. The rhizosphere effect also showed a similar pattern of seasonal dynamics with the input of the root exudates, summer(July) is the highest, while winter(December) is the lowest. (3)By correlation analysis, there was a positive linear correlation between the DOC and TN rates in root exudates, and the rhizosphere effect of MBC, MBN rhizosphere effect and soil enzyme activity implied that the root secretion input was an important factor that could drive the activity of the rhizosphere microorganisms, and reveal the seasonal response of the input of the tree roots to the process and function of the rhizosphere microbial process. Future research should be strengthened in the coupling effect and mechanism about the root secretion input and the process of soil biogeochemical cycle.

Key words: Cercidiphyllum japonicum Sieb. plantation, root exudates, rhizosphere microorganisms, soil enzymes, seasonal changes

中图分类号:

Q948.122

王小平, 肖肖, 唐天文, 黎云祥, 肖娟. 连香树人工林根系分泌物输入季节性变化及其驱动的根际微生物特性研究[J]. 植物研究, 2018, 38(1): 47-55.

WANG Xiao-Ping, XIAO Xiao, TANG Tian-Wen, LI Yun-Xiang, XIAO Juan. Seasonal Changes of the Input of Root Exudates and Its Driving Characteristics of Rhizosphere Microbe in a Cercidiphyllum japonicum Sieb. Plantation[J]. Bulletin of Botanical Research, 2018, 38(1): 47-55.

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