刚毛柽柳ThPP2C基因的克隆和表达分析

doi:10.7525/j.issn.1673-5102.2017.03.010

植物研究 ›› 2017, Vol. 37 ›› Issue (3): 395-401.doi: 10.7525/j.issn.1673-5102.2017.03.010

刚毛柽柳ThPP2C基因的克隆和表达分析

王培龙, 刘中原, 张腾倩, 唐绯绯, 曲冠证, 高彩球

东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040

收稿日期:2017-01-03 出版日期:2017-05-15 发布日期:2017-06-03
通讯作者: 高彩球,E-mail:gaocaiqiu@nefu.edu.cn E-mail:gaocaiqiu@nefu.edu.cn
作者简介:王培龙(1990-),男,博士研究生,主要从事林木遗传育种研究。
基金资助:
黑龙江省教育厅科学技术研究项目（12523017）

Cloning and Expression Analysis of ThPP2C Gene from Tamarix hispida

WANG Pei-Long, LIU Zhong-Yuan, ZHANG Teng-Qian, TANG Fei-Fei, QU Guan-Zheng, GAO Cai-Qiu

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040

Received:2017-01-03 Online:2017-05-15 Published:2017-06-03
Supported by:
Science and technology research project of Heilongjiang Provincial Department of Education(12523017)

摘要/Abstract

摘要： 蛋白磷酸酶2C（PP2C）是一类丝氨酸/苏氨酸蛋白磷酸酶，在体内以单体形式存在，由它催化的可逆磷酸化反应是细胞信号转导的重要组成部分，这一过程几乎参与所有的生理和病理过程。本研究从柽柳中克隆获得一条PP2C基因，命名为ThPP2C。该基因开放读码框（ORF）长849 bp，编码282氨基酸，推测蛋白质分子量为30.54 kDa，理论等电点为7.13。qRT-PCR结果显示，0.4 mol·L^-1 NaCl、20%（w/v） PEG₆₀₀₀、150 μmol·L^-1 ABA和100 μmol·L^-1 JA胁迫处理后，ThPP2C基因在刚毛柽柳根和叶中的表达均发生了明显改变，但时空表达模式不完全相同。NaCl、ABA和JA处理后ThPP2C基因在叶中都表现为下调表达。而根中，NaCl和JA胁迫后主要表现为上调表达。ABA处理早期表达下调，随后表达量逐渐增加。与其他3种胁迫不同的是，PEG₆₀₀₀处理后ThPP2C基因在根中明显下调，而在叶中胁迫早期表现不明显，48 h后被高度诱导。表明ThPP2C基因可能参与了刚毛柽柳高盐、干旱以及激素处理的适应过程，但其功能还有待进一步验证。

关键词: 蛋白磷酸酶2C(PP2C), 刚毛柽柳, 盐胁迫, ABA

Abstract: Protein phosphatase 2C(PP2C) is a kind of serine/threonine protein phosphatase, which is present in the body as monomer. The reversible phosphorylation by PP2C catalytic is an important component of the cell signal transduction, which is involved almost all the physiological and pathological processes. A novel PP2C gene(ThPP2C) was isolated from Tamarix hispida by RT-PCR. The opening reading frame(ORF)length of ThPP2C was 869 bp, encoding 282 amino acid. The relative molecular weight of ThPP2C protein was 30.54 kD, with isoelectric point 7.13. By the real-time RT-PCR, the expressions of ThPP2C were obviously difference in roots and leaves of T.hispida under the treatments(0.4 mol·L^-1 NaCl, 20%(w/v) PEG₆₀₀₀, 150 μmol·L^-1 ABA or 100 μmol·L^-1 JA) compared to the control. The expression patterns of ThPP2C were not exactly similarity among these treatments. Particularly, the expression of ThPP2C in leaves of T.hispida after the treatments of NaCl, ABA and JA all were down-regulated. However, it was mainly up-regulated in roots under the treatments of NaCl and JA. With the treatment of ABA, the ThPP2C expression was down-regulated at the early stage and then gradually increased. Different with other three stress, under the treatment of PEG₆₀₀₀, the expressions of ThPP2C were obviously down-regulated in roots. It was not obviously different at the early stress stage in leaves until 48 h, when it was highly induced. Therefore, ThPP2C may play an important role in T.hispida to response the high salt, drought stress and hormones.

Key words: PP2C, Tamarix hispida, salt stress, ABA treatment

中图分类号:

王培龙, 刘中原, 张腾倩, 唐绯绯, 曲冠证, 高彩球. 刚毛柽柳ThPP2C基因的克隆和表达分析[J]. 植物研究, 2017, 37(3): 395-401.

WANG Pei-Long, LIU Zhong-Yuan, ZHANG Teng-Qian, TANG Fei-Fei, QU Guan-Zheng, GAO Cai-Qiu. Cloning and Expression Analysis of ThPP2C Gene from Tamarix hispida[J]. Bulletin of Botanical Research, 2017, 37(3): 395-401.

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刚毛柽柳ThPP2C基因的克隆和表达分析

Cloning and Expression Analysis of ThPP2C Gene from Tamarix hispida

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