外源铜胁迫对长春花幼苗生物碱的影响

doi:10.7525/j.issn.1673-5102.2016.03.009

植物研究 ›› 2016, Vol. 36 ›› Issue (3): 374-379.doi: 10.7525/j.issn.1673-5102.2016.03.009

外源铜胁迫对长春花幼苗生物碱的影响

林枫¹, 高媚娇², 于博帆², 陈华峰²

1. 辽宁省林业调查规划院, 沈阳 110122;
2. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040

收稿日期:2015-11-21 出版日期:2016-05-15 发布日期:2016-05-20
通讯作者: 陈华峰,E-mail:biginsect2002@163.com E-mail:biginsect2002@163.com
作者简介:林枫(1977-),男,高级工程师,主要从事植物和林业调查研究工作。
基金资助:
国家自然科学基金青年科学基金项目(31400337)

Effects of Copper Stress on the Alkaoids of Catharanthus roseus

LIN Feng¹, GAO Mei-Jiao², YU Bo-Fan², CHEN Hua-Feng²

1. Liaoning Forestry Survey and Planning Institute, Shenyang 110122;
2. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040

Received:2015-11-21 Online:2016-05-15 Published:2016-05-20

摘要/Abstract

摘要： 以药用植物长春花为实验材料,采用珍珠岩为培养基质的营养方式培养,初步研究了长春花幼苗对铜胁迫的生长响应、吸收积累、基因表达及生物碱合成的特性。研究结果表明,在外源铜胁迫下,药用植物长春花幼苗根和叶干重表现为低浓度的上升和高浓度的降低,并在300μmol·L^-1Cu时达到峰值,与对照差异显著(P<0.05)。而幼苗的根系耐性指数先上升后下降,在200μmol·L^-1Cu时达到峰值。Cu金属进入长春花幼苗体内绝大部分积累在根部。长春花叶片中含有的长春碱在外源铜胁迫条件下其含量有一定程度上的增加,并且随着处理浓度的增加使之呈现出规律性变化,在300μmol·L^-1Cu时达到峰值,甚至到达CK的2.7倍。结果可为进一步验证长春花是否可以作为一种新的Cu超富集植物提供参考依据。

关键词: 长春花, 铜胁迫, 长春碱, Cu超富集植物

Abstract: With Catharanthus roseus, we used perlite as the medium quality nutritional way to study the effects of copper stresson theresponses of C.roseus seedling growth, accumulation, gene expression and characteristics of alkaloid synthesis. The dry weight of root and leaf of C.roseus under exogenous Cu stress levels was increased under low concentration,decreased under high concentration,and reached peak at 300 μmol·L^-1, with significant difference compared with the control(P<0.05). The root tolerance index of seedlings was increased firstly, then decreased, and reached the peak at 200 μmol·L^-1 of Cu. Cu into C.roseus seedlings was mostly accumulated in roots. But under the stress of exogenous Cu, C.roseus blade parameters, such as the accumulation of dry matter, was improved significantly, by presenting a regular change the concentration was increased, and reached the peak at 300 μmol·L^-1 Cu, even reaching as much as 2.7 times thanthe CK. The C.roseus hyperaccumulation plants can be usedas further reference basisfor Cu accumulation.

Key words: Catharanthus roseus, copper stress, vinblastine, hyperaccumulation plants of Cu

中图分类号:

S567

林枫, 高媚娇, 于博帆, 陈华峰. 外源铜胁迫对长春花幼苗生物碱的影响[J]. 植物研究, 2016, 36(3): 374-379.

LIN Feng, GAO Mei-Jiao, YU Bo-Fan, CHEN Hua-Feng. Effects of Copper Stress on the Alkaoids of Catharanthus roseus[J]. Bulletin of Botanical Research, 2016, 36(3): 374-379.

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外源铜胁迫对长春花幼苗生物碱的影响

Effects of Copper Stress on the Alkaoids of Catharanthus roseus

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