胞外ATP通过H2O2对菜豆叶片光系统Ⅱ光化学特性的系统性影响

doi:10.7525/j.issn.1673-5102.2016.03.008

植物研究 ›› 2016, Vol. 36 ›› Issue (3): 368-373.doi: 10.7525/j.issn.1673-5102.2016.03.008

胞外ATP通过H₂O₂对菜豆叶片光系统Ⅱ光化学特性的系统性影响

田武英, 焦青松, 石岱龙, 孙坤, 贾凌云, 冯汉青

西北师范大学生命科学学院, 兰州 730070

收稿日期:2015-10-19 出版日期:2016-05-15 发布日期:2016-05-20
通讯作者: 贾凌云,E-mail:lingyunjia1982@126.com E-mail:lingyunjia1982@126.com
作者简介:田武英(1989-),女,硕士研究生,主要从事植物生理生态研究。
基金资助:
国家自然科学基金资助项目(31260059和30900105);教育部科学技术研究重点资助项目(211190);甘肃省财政厅高校基本科研业务资助项目

Extracellular ATP Exert Systemic Effects on Photosystem Ⅱ Photochemistry of Kidney Bean(Phaseolus vulgaris) Leaf through a H₂O₂ Signalling Pathway

TIAN Wu-Ying, JIAO Qing-Song, SHI Dai-Long, SUN Kun, JIA Ling-Yun, FENG Han-Qing

College of Life Science, Northwest Normal University, Lanzhou 730070

Received:2015-10-19 Online:2016-05-15 Published:2016-05-20

摘要/Abstract

摘要： 研究了细胞外ATP对菜豆叶片光系统Ⅱ(PSⅡ)光化学特性的系统性影响及可能的内在机制。研究发现,用外源ATP处理菜豆叶片的一侧,处理侧和同叶未处理侧潜在最大光化学效率(F_v/F_m)、非调节性能量耗散量子产量(Y(NO))与对照相比无显著变化;而实际光化学效率(Y(Ⅱ))、电子传递速率(ETR)和光化学猝灭系数(qP)与对照相比显著上升,但非光化学猝灭系数(qN)和调节性能量耗散量子产量(Y(NPQ))则显著下降。表明了细胞外ATP能够系统性地提高菜豆叶片PSⅡ光化学效率。用外源ATP处理菜豆叶片的一侧,检测叶片处理侧与同叶未处理侧过氧化氢的含量,发现与对照相比,叶片处理侧与未处理侧过氧化氢含量均显著上升。用外源过氧化氢(H₂O₂)处理菜豆叶片一侧也导致了处理侧与同叶未处理侧Y(Ⅱ)、ETR和qP的显著上升,以及qN和Y(NPQ)的显著下降。因此,过氧化氢和ATP能够对处理侧与同叶未处理侧叶片的光化学特性产生相似的影响。进一步研究表明,用过氧化氢的淬灭剂N,N'-二甲基硫脲(DMTU,dimethylthiourea)可以减弱ATP对菜豆叶片处理侧与未处理侧Y(Ⅱ)、ETR、qP、qN和Y(NPQ)产生的影响。这些结果表明:菜豆叶片局部细胞外ATP的增加可加强邻近组织PSⅡ的光化学效率,并且胞外ATP对叶片PSⅡ光化学特性的这种系统性影响依赖于H₂O₂调节的下游信号途径。

关键词: 叶绿素荧光, 细胞外ATP, 过氧化氢(H₂O₂), 菜豆, 系统性

Abstract: We studied the systemic effects of extracellular ATP(eATP) on the Photosystem Ⅱ photochemistry of kidney bean(Phaseolus vulgaris) leaf and possible mechanism for this effect. Treatment of a half of a bean leaf with exogenous ATP had no significant effect on the maximal photochemical efficiency of photosystem Ⅱ(F_v/F_m), and the quantum yield of non-regulated energy dissipation(Y(NO)) of the treated half and the untreated, opposite half of the same leaf. However, this treatment significantly increased the values of photochemical efficiency(Y(Ⅱ)), the rate of photosynthetic electron transport(ETR), and photochemical quenching(qP), while the values of the non-photochemical quenching(qN) and the quantum yield of regulated energy dissipation(Y(NPQ)) were significantly decreased by exogenous 1 mmol·L^-1 ATP. These observations indicate that eATP can function in systematically enhancing the PSⅡ photochemistry of kidney bean leaves. Treatment of a half of a bean leaf with exogenous ATP, we tested the change of the oxidation hydrogen content of the treated half and the untreated, opposite half of the same leaf. Compared to the control, hydrogen peroxide content of the treated half and the untreated, opposite half of the same leaf were significantly increased. Similar to the effects of exogenous ATP, treatment with exogenous H₂O₂ at 1 mmol·L^-1 also led to a significant increase in Y(Ⅱ), qP and ETR and a significant decrease in qN and Y(NPQ) of the treated half and the untreated, opposite half of the same leaf. DMTU(dimethylthiourea, a scavenger of H₂O₂) weakened the effects of exogenous ATP on the Y(Ⅱ)), qP, ETR, qN and Y(NPQ). These observations indicate that an increase in eATP concentration of partial region of bean leaf can enhance the PSⅡ photochemistry of the adjacent tissues, and such function of eATP could be dependent of H₂O₂.

Key words: chlorophyll fluorescence, extracellular ATP, exogenous H₂O₂, kidney bean(Phaseolus vulgaris), systemic

中图分类号:

S643.1

田武英, 焦青松, 石岱龙, 孙坤, 贾凌云, 冯汉青. 胞外ATP通过H₂O₂对菜豆叶片光系统Ⅱ光化学特性的系统性影响[J]. 植物研究, 2016, 36(3): 368-373.

TIAN Wu-Ying, JIAO Qing-Song, SHI Dai-Long, SUN Kun, JIA Ling-Yun, FENG Han-Qing. Extracellular ATP Exert Systemic Effects on Photosystem Ⅱ Photochemistry of Kidney Bean(Phaseolus vulgaris) Leaf through a H₂O₂ Signalling Pathway[J]. Bulletin of Botanical Research, 2016, 36(3): 368-373.

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胞外ATP通过H₂O₂对菜豆叶片光系统Ⅱ光化学特性的系统性影响

Extracellular ATP Exert Systemic Effects on Photosystem Ⅱ Photochemistry of Kidney Bean(Phaseolus vulgaris) Leaf through a H₂O₂ Signalling Pathway

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