金丝桃苷双水相提取工艺研究

doi:10.7525/j.issn.1673-5102.2013.03.018

植物研究 ›› 2013, Vol. 33 ›› Issue (3): 367-370.doi: 10.7525/j.issn.1673-5102.2013.03.018

金丝桃苷双水相提取工艺研究

金彦^1,2；姚晓慧^1,2；段明慧^1,2；张东阳^1,2；付玉杰^1,2*

1.东北林业大学森林植物生态学教育部重点实验室，哈尔滨　150040；2.东北林业大学生物制剂教育部工程研究中心，哈尔滨　150040

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-05-20 发布日期:2013-05-20
通讯作者: 付玉杰
基金资助:

Aqueous Two-phase System(ATPS) Extraction of Hyperin

JIN Yan;YAO Xiao-Hui;DUAN Ming-Hui;ZHANG Dong-Yang;FU Yu-Jie;*

1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin　150040；2.Engineering Research Center of Forest Bio-preparation,Ministry of Education,Northeast University,Harbin　150040

Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
Contact: FU Yu-Jie
Supported by:

摘要/Abstract

摘要： 以鹿蹄草为原料，利用微波双水相辅助提取技术进行提取，在单因素实验的基础上对提取条件进行了考察，根据BBD(BoxBehnken Design)实验设计原理，采用3因素3水平的响应面分析法，以鹿蹄草中金丝桃苷(hyperin)为指标，对提取过程进行优化，得到最佳工艺参数为:提取温度50℃,液固比36∶1，(NH₄)₂SO₄浓度22%，提取时间22 min，微波功率300 W，提取次数3次。在最佳提取条件下，金丝桃苷的提取率可达0.982 mg·g^-1，纯度可以达0.443%。

关键词: 鹿蹄草, 金丝桃苷, 双水相辅助提取, Box-Behnken Design设计

Abstract: Based on the preliminary experiments, a three-variable, three-level Box-Behnken design was performed to study the aqueous two-phase system (ATPS) extraction of hyperin from Pyrola calliantha H. Andr.. The optimum parameters were as follows: extraction temperature 50℃, liquid /solid ratio 36∶1, (NH₄)₂SO₄ concentration 22%, extraction time 22 min, extraction power 300 W and three extraction cycles. Under the optimal conditions, the average extraction yield of hyperin was 0.982 mg·g^-1 and the purity was 0.443%. This study is significant for the application of an aqueous two-phase system (ATPS) extraction technology and utilization of P.calliantha H. Andr..

Key words: Pyrola calliantha H.Andr., hyperin, aqueous two-phase system(ATPS) extraction, Box-Behnken design

中图分类号:

S567.23

金彦;姚晓慧;段明慧;张东阳;付玉杰;*. 金丝桃苷双水相提取工艺研究[J]. 植物研究, 2013, 33(3): 367-370.

JIN Yan;YAO Xiao-Hui;DUAN Ming-Hui;ZHANG Dong-Yang;FU Yu-Jie;*. Aqueous Two-phase System(ATPS) Extraction of Hyperin[J]. Bulletin of Botanical Research, 2013, 33(3): 367-370.

金丝桃苷双水相提取工艺研究

Aqueous Two-phase System(ATPS) Extraction of Hyperin

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