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植物研究 ›› 2013, Vol. 33 ›› Issue (3): 367-370.doi: 10.7525/j.issn.1673-5102.2013.03.018

• 论文 • 上一篇    下一篇

金丝桃苷双水相提取工艺研究

金彦1,2;姚晓慧1,2;段明慧1,2;张东阳1,2;付玉杰1,2*   

  1. 1.东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040;2.东北林业大学生物制剂教育部工程研究中心,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-05-20 发布日期:2013-05-20
  • 通讯作者: 付玉杰
  • 基金资助:
     

Aqueous Two-phase System(ATPS) Extraction of Hyperin

JIN Yan;YAO Xiao-Hui;DUAN Ming-Hui;ZHANG Dong-Yang;FU Yu-Jie;*   

  1. 1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin 150040;2.Engineering Research Center of Forest Bio-preparation,Ministry of Education,Northeast University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
  • Contact: FU Yu-Jie
  • Supported by:
     

摘要: 以鹿蹄草为原料,利用微波双水相辅助提取技术进行提取,在单因素实验的基础上对提取条件进行了考察,根据BBD(BoxBehnken Design)实验设计原理,采用3因素3水平的响应面分析法,以鹿蹄草中金丝桃苷(hyperin)为指标,对提取过程进行优化,得到最佳工艺参数为:提取温度50℃,液固比36∶1,(NH4)2SO4浓度22%,提取时间22 min,微波功率300 W,提取次数3次。在最佳提取条件下,金丝桃苷的提取率可达0.982 mg·g-1,纯度可以达0.443%。

关键词: 鹿蹄草, 金丝桃苷, 双水相辅助提取, Box-Behnken Design设计

Abstract: Based on the preliminary experiments, a three-variable, three-level Box-Behnken design was performed to study the aqueous two-phase system (ATPS) extraction of hyperin from Pyrola calliantha H. Andr.. The optimum parameters were as follows: extraction temperature 50℃, liquid /solid ratio 36∶1, (NH4)2SO4 concentration 22%, extraction time 22 min, extraction power 300 W and three extraction cycles. Under the optimal conditions, the average extraction yield of hyperin was 0.982 mg·g-1 and the purity was 0.443%. This study is significant for the application of an aqueous two-phase system (ATPS) extraction technology and utilization of P.calliantha H. Andr..

Key words: Pyrola calliantha H.Andr., hyperin, aqueous two-phase system(ATPS) extraction, Box-Behnken design

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