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植物研究 ›› 2012, Vol. 32 ›› Issue (6): 646-650.doi: 10.7525/j.issn.1673-5102.2012.06.002

• 论文 • 上一篇    下一篇

香果树花及胚胎发育的细胞学研究

李利平;李争艳;王玉兵;梁宏伟;陈发菊*   

  1. 三峡大学生物技术研究中心,宜昌 443002
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-11-20 发布日期:2012-11-20
  • 通讯作者: 陈发菊
  • 基金资助:
     

Cytology Research on the Flower and Embryo Development of Emmenopterys henryi Oliv

LI Li-Ping;LI Zheng-Yan;WANG Yu-Bing;LIANG Hong-Wei;CHEN Fa-Ju*   

  1. Biotechnology Research Center,China Three Gorges University,Yichang 443002
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-20 Published:2012-11-20
  • Contact: CHEN Fa-Ju
  • Supported by:
     

摘要: 香果树为茜草科香果树属单种属植物,是我国特种古老珍稀濒危树种。对香果树大小孢子发生及胚胎发育过程进行了细胞学观察,观察结果表明:香果树子房两室,中轴胎座,胚珠多数,倒生,单珠被,厚珠心。大孢子四分体排列成线性,靠近合点端的大孢子发育成功能大孢子,珠孔端的3个退化,胚囊发育为蓼型,典型的七胞八核胚囊。雄蕊浅黄色,蝶形花粉囊,小孢子四分体成四面体型,成熟的花粉为二胞花粉。胚胎发育过程经历了合子、原胚、球形胚、心形胚、鱼雷胚5个阶段,种子成熟时,胚发育未完全成熟。本文在香果树大小孢子发生发育切片的观察中未发现异常现象,胚胎发育停留在鱼雷胚时期。

关键词: 香果树, 大孢子, 小孢子, 胚胎发育

Abstract: Emmenopterys henryi Oliv, one of the China’s unique species, belongs to Rubiaceae. It is an endangered endemic single species genus plant. A detailed morphological description of megasporogenesis, microsporogenesis, and the development of the embryo in the rare endangered plant E.henryi is presented. The ovule is anatropous, single-integument, and crassinucellate. The megaspore mother cells undergo miosis and develop into a linear tetrad. The large one at chalazal end is functional megaspore and developed into a polygonum type of embryo sac after the third mitotic division. Each anther of E.henryi is with four sacs. The meioses of microspore mother cell is normal and cytokinesis is modified simultaneous type, and tetrads are tetrahedral. The pollen grains are monocolpate and two celled at shedding. The embryogeny passed through zygote, proembryo, globular embryo, heart and torpedo stages. When the seeds are ripe, embryo is not completely mature. Through the study we found that there is no abnormal phenomenon in the megasporogenesis, microsporogenesis process and the embryo stays in torpedo stage.

Key words: Emmenopterys henryi Oliv, megasporogenesis, microsporogenesis, embryo development

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