关键词: 樟, 龙脑樟, 组织培养, 工厂化生产

Abstract: The techniques of tissue culture of Cinnamomum camphora(Linn.) Pves were developed from the explants taken from young sprouting branches at the base of plus tree with high contents of borneo camphor. The results showed that the adventitious buds were induced at the induction rate of 93% on modified MS supplemented with BA 2.0 mg·L^-1 and NAA 0.1 mg·L^-1. The modified MS adding BA 2.0 mg·L^-1 and NAA 0.05 mg·L^-1 was the optimized subculture medium with multiplication coefficient being 5.57 and the growth cycle being 30 d. The shoots grew well under the conditions of temperature 25℃, illumination intensity 3 000 lx and illumination time 11 h. They rooted in 12 d on 1/2 MS adding IBA 0.5 mg·L^-1, IAA 0.4 mg·L^-1 and sucrose 20 g·L^-1 with rooting rate reaching up to 97.33%, and the number of roots being 3 to 5. The plantlets were transplanted in mixture medium with peat and perlite(3∶1), and the survival rate was 86.2%. These techniques could be used in industrialized seedling production for the chemical form of this species.

Key words: Cinnamomum camphora, borneol camphor, tissue culture, industrialization production