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植物研究 ›› 2010, Vol. 30 ›› Issue (6): 758-762.doi: 10.7525/j.issn.1673-5102.2010.06.024

• 论文 • 上一篇    下一篇

防己抑藻效应及其化感物质的HPLC分析

柴民伟;石福臣*;马妍   

  1. 南开大学生命科学学院,天津 300071
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
  • 通讯作者: 石福臣
  • 基金资助:
     

Inhibitory Effect of Algae Growth and HPLC Analysis of Allelochemicals from Stephania tetrandra

CHAI Min-Wei;SHI Fu-Chen*;MA Yan   

  1. College of Life Sciences,Nankai University,Tianjin 300071
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
  • Contact: SHI Fu-Chen
  • Supported by:
     

摘要: 通过常温水浸提方法获得12种药用植物浸提液,并分别研究其抑藻效应。结果显示:防己水浸提物(相对浓度1 g·L-1)抑制蛋白核小球藻的效应最强,最大比生长率为-0.28 d-1。防己甲醇浸提物对蛋白核小球藻的抑藻效应显著,且持续时间长,最低有效抑藻浓度为30 mg·L-1。HPLC鉴定结果显示:防己水浸提液和甲醇浸提液中主要含有防己诺林碱、粉防己碱等生物碱类化学成分,防己对治理水华发生有较大的应用前景。

关键词: 药用植物, 水华, 防己, 蛋白核小球藻, 高效液相色谱

Abstract: Aqueous extracts of 12 kinds of medicinal plants were obtained by soak extraction, and their inhibitory effects on algae growth were studied. The results showed that: when the relative concentration was 1 g·L-1, aqueous extracts of Stephania tetrandra had the strongest inhibitory effect on the growth of Chlorella pyrenoidosa, and the maximum specific growth rate was -0.28 d-1. The methanol extract of S.tetrandra had significant and long-lasting inhibitory effect on the growth of C.pyrenoidosa and the lowest effective concentration in inhibiting algae was 30 mg·L-1.The identification results of HPLC showed that: the main constituents in the extracts were alkaloids such as fangchinoline and tetrandrine, etc.S.tetrandra had great prospect of application in controlling water bloom.

Key words: medicinal plant, water bloom, Stephania tetrandra, Chlorella pyrenoidosa, HPLC

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