关键词: 无融合生殖, 甜菜无融合生殖系M14, mRNA差异展示技术

Abstract: A monosomic addition line in sugar beet, which was designated as M₁₄ was obtained by the wide cross between cultivated sugar beet (Beta vulgaris L.) and Beta corolliflora Zoss. M₁₄ contained the 18 normal chromosomes of sugar beet plus the B. corolliflora chromosome 9. This alien chromosome in M₁₄ had an average transmission frequency of 96.5% through eggs, the reason it had so high transmission frequency was that it had apomixis genes. By mRNA differential display method, the study compared gene expression at the stage of meiosis in florescence between M₁₄ and cultivated sugar beet (Beta vulgaris L.) A_2Y which can carry out normal sexual reproduction. The anchored primer were GT₁₅A, GT₁₅G, GT₁₅C,and twenty arbitrary primers were used. By RT-PCR, six positive specific cDNA fragments were obtained. Analysis was performed on NCBI, and comparison the similarity with published sequences was carried out using the BLASTx program. These cDNA fragments were the candidate fragments to clone the apomixis genes.

Key words: apomixis, apomictic lines M₁₄ in sugar beet, mRNA differential display