紫茎泽兰花蕾发育过程中类Beclin1基因的克隆表达分析

doi:10.7525/j.issn.1673-5102.2007.04.017

植物研究 ›› 2007, Vol. 27 ›› Issue (4): 460-464.doi: 10.7525/j.issn.1673-5102.2007.04.017

紫茎泽兰花蕾发育过程中类Beclin1基因的克隆表达分析

王　敏;刘　英;李思滨;姜　洋;祁长青;王　俐;祖元刚^*

东北林业大学森林植物生态学教育部重点实验室，哈尔滨　150040

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-07-20 发布日期:2007-07-20
通讯作者: 祖元刚
基金资助:

Cloning and Characterization of Beclin1-like Gene in Eupatorium adenophorum Buds

WANG Min;LIU Ying;LI Si-Bin;JIANG Yang;QI Chang-Qing;WANG Li;ZU Yuan-Gang*

Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin　150040

Received:1900-01-01 Revised:1900-01-01 Online:2007-07-20 Published:2007-07-20
Contact: ZU Yuan-Gang
Supported by:

摘要/Abstract

摘要： 以紫茎泽兰不同发育时期花蕾为实验材料，通过光学显微观察、DNA ladder检测表明紫茎泽兰花蕾发育过程中绒毡层有PCD现象发生，自紫茎泽兰花蕾中克隆长为679 bp的Beclin1 cDNA基因部分片段，同烟草叶片的Beclin1基因序列（AY701316）同源性为98%，通过Northern blotting分析，在紫茎泽兰花蕾发育过程中细胞程序性死亡（PCD）相关Beclin1基因表达在花蕾发育第Ⅱ期和Ⅲ期较为旺盛，且在花蕾发育第Ⅱ期最强烈。本研究首次克隆并证实紫茎泽兰花蕾Beclin1基因与PCD有一定的相关性，为分析紫茎泽兰入侵机理提供新的思路和手段。

关键词: 紫茎泽兰, 花蕾, 细胞程序性死亡（PCD）, Beclin1基因, Northern blotting分析

Abstract: It was found in the present study that programmed cell death (PCD) took place during the tapetum degenerating and pollen developing by microscopical observation and DNA ladder detection in Eupatorium adenophorum buds. Simultaneously, Beclin1-like gene was cloned from E. adenophorum buds at four different growth stages by RT-PCR method. The partial Beclin1-like gene (679 bp) was 98% similar to that of tobacco leaves (AY701316). Northern blotting showed that the expression of Beclin1-like gene in the stage Ⅱ was the strongest. The Beclin1-like gene was cloned in E. adenophorum buds for the first time and some relationships between the Beclin1-like Gene and PCD during the procreation of E. adenophorum were demonstrated. In addition, the results provided a new idea to further explore the invading mechanism of E. adenophorum.

Key words: Eupatorium adenophorum, buds, programmed cell death(PCD), Beclin1, Northern blotting analysisaf

中图分类号:

王　敏;刘　英;李思滨;姜　洋;祁长青;王　俐;祖元刚*. 紫茎泽兰花蕾发育过程中类Beclin1基因的克隆表达分析[J]. 植物研究, 2007, 27(4): 460-464.

WANG Min;LIU Ying;LI Si-Bin;JIANG Yang;QI Chang-Qing;WANG Li;ZU Yuan-Gang*. Cloning and Characterization of Beclin1-like Gene in Eupatorium adenophorum Buds[J]. Bulletin of Botanical Research, 2007, 27(4): 460-464.

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紫茎泽兰花蕾发育过程中类Beclin1基因的克隆表达分析

Cloning and Characterization of Beclin1-like Gene in Eupatorium adenophorum Buds

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