关键词: 水稻, 遗传转化, 反义蜡质基因, 直链淀粉含量, 蛋白质含量

Abstract: It was reported that there was a negative correlation between amylose content and protein content in the rice seeds. In this research anti-waxy-gus fusion gene was transferred into the rice immature embryo callus by Agrobacterium infection. Eight transgenic plants (fertile) were obtained. That the anti-waxy-gus fusion gene had been integrated into the eight transgenic plants’ genomes was testified by PCR and Southern blot tests and the anti-waxy-gus fusion gene could be expressed correctly by GUS-dyeing in grain of rice. Sixteen homozygous transgenic plants in anti-waxy-gus fusion gene position were obtained through GUS analyses. Amylose and protein contents in the sixteen plants’ seeds were analyzed. The results indicated:(1) There were significant negative correlations between protein contents and amylose contents in the sixteen transgenic plants’ seeds; (2) Amylose average content and protein average content in the control were respectively 13.4% and 9.5%. The amylose content in one transgenic plant was lowest (11.4%), at the same time its protein content was highest (13.5%). This shows that tasting quality and nutritional quality of rice seeds are improved by introducing anti-waxy gene.

Key words: rice, transformation, anti-waxy gene, amylose content, protein content