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植物研究 ›› 2004, Vol. 24 ›› Issue (2): 245-247.

• 论文 • 上一篇    下一篇

离体条件下暴马丁香切开种子的萌发

刘华英, 沈海龙, 黄剑, 王红梅, 邢朝斌   

  1. 东北林业大学林学院, 哈尔滨 150040
  • 收稿日期:2003-11-08 出版日期:2004-06-15 发布日期:2016-06-14
  • 作者简介:刘华英(1976-), 女, 硕士, 主要从事遗传育种研究。

In vitro germination of cut seeds of Syringa reticulata var .mandshurica

LIU Hua-Ying, SHEN Hai-Long, HUANG Jian, WANG Hong-Mei, XING Zhao-Bin   

  1. College of Forestry, No rtheast Fo restry University, Harbin 150040
  • Received:2003-11-08 Online:2004-06-15 Published:2016-06-14

摘要: 分别以三种催芽处理10、20、40 d的暴马丁香种子进行离体培养试验,对种子做三种切割处理,中间切、两端切和完整种子。结果表明:采用切开种子的方法进行离体培养可提高种子发芽率,中间切种子的萌发最好,且在培养10 d后发芽率即达到最高值;经过40 d催芽处理的种子优于未经催芽和只进行20 d催芽处理的种子;切开种子以MS培养基附加5 mg/L BA或5 mg/LBA+0.1 mg/L IBA为最适培养基。

关键词: 暴马丁香, 离体培养, 种子发芽率

Abstract: Seeds of Syringa reticulata var.mandshurica stratified for 10,20 and 40 days were in vitro cultured.Before incubation,three different treatments of seeds were conducted:intact,middlecut,two ends-cut.The results showed that germination percentage of cut seed was enhanced,middle-cut was the best and achieved maximum germination after 10 days culture;germination of seeds treated 40 days was better than that of the seeds with other two treatments.In vitro germination of seeds with 40 days stratification was best on MS medium with 5 mg/L BA or BA 5 mg/L BA+0.1 mg/L IBA.

Key words: Syringa reticulata var .mandshurica, in vi tro culture, seed germination percentage